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洛伐他汀对氧化修饰低密度脂蛋白刺激U937细胞缺氧诱导因子-1α表达的影响 被引量：1

Effects of lovastatin on expression of hypoxia inducible factor 1 alpha on U937 cell induced by oxidized low density lipoprotein
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摘要 目的:研究氧化修饰低密度脂蛋白(ox-LDL)刺激U937细胞表达缺氧诱导因子-1α(HIF-1α)的变化,并观察洛伐他汀对其的影响。方法:用油红染色的方法检测泡沫细胞的形成,采用实时定量PCR(Real-timePCR)及蛋白印记杂交技术(W estern blotting)检测细胞HIF-1α表达的变化。结果:未加ox-LDL刺激的U937细胞未见泡沫细胞形成;U937细胞加入ox-LDL刺激24 h后,全部转化为泡沫细胞。ox-LDL刺激下,U937细胞HIF-1α表达量显著升高,加入洛伐他汀后,HIF-1α的表达量显著降低,随剂量增加,抑制作用显著增强,有明显的剂量依赖关系。结论:ox-LDL氧化损伤导致巨噬细胞HIF-1α高表达,洛伐他汀可抑制U937细胞HIF-1α的表达,抑制泡沫细胞的形成。 Objective：To study the effects of expression of hypoxia inducible 1 alpha （ HIF-1 α） on U937 cell stimulated by oxidized low density lipoprotein（ox-LDL） and the influence of lovastatin. Methods： Foam cell formation was tested by oil red O-stain a- nalysis, and expression of HIF-1α was measured by the method of Real-time PCR and Western blotting. Results： Foam cell was not formed by U937 cell without ox-LDL stimulating; U937 cell with ox-LDL stimulating for 24 h was transformed to foam cell. After treated with ox-LDL, the expression of HIF-1 αwas markedly increased on U937 cells; with the combined treatment of lovastafin ox-LDL, the expression of HIF-1 αwas significantly decreased, and this inhibitory effect was dose dependant. Conclusion：Lovasatin could inhibit both the expression of HIF-1α on U937 and the foam cell formation induced by ox-LDL.
作者 蒋国军 张黎 李铁军 邱彦 楼宜嘉
机构地区 浙江萧山医院药剂科 中国人民解放军第二军医大学药学院 浙江大学药学院
出处 《药学实践杂志》 CAS 2009年第3期170-173,共4页 Journal of Pharmaceutical Practice
关键词 洛伐他汀 氧化修饰低密度脂蛋白 缺氧诱导因子 lovastatin oxidized low density lipoprotein hypoxia inducible factor 1
分类号 R96 [医药卫生—药理学]
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参考文献11

  • 1k Cramer T, Johnson RS. A novel role for the hypoxia inducible transcription factor HIF-1α: critical regulation of inflammatory cell fuction [ J ]. Cell cycle, 2003,2 ( 3 ) : 192.
  • 2Cramer T, Yamanishi Y, Clausen BE, et al. HIF-1 alpha is essential for myeloid cell - mediated inflammation [ J ]. Cell, 2003,112(5) :645.
  • 3Dichtl W, Dulak J, Frick M. et al. HMG-CoA reductase inhibitors regulate inflammatory Ttranscription factors in human endothelial and vascular smooth muscle cells[ J]. Arterioscler Thromb Vasc Biol, 2003, 23 ( 1 ) :58.
  • 4Wilson SH, Herrmann J, Lerman LO, et al. Simvastatin preserves the structure of coronary adventitial vasa vasorum in experimental hypercholesterolemia independent of lipid lowering [ J ]. Circulation,2002,105 (4) :415.
  • 5Steinberg, D. Low density lipoprotein oxidation and its pathobiological significance [ J ]. J Biol Chem, 1997,272 (34) :20963.
  • 6Semenza GL, Agani F, Feldser D, et al. Hypoxia , HIF-1, and the pathophysiology common human diseases [ J ]. Adv Exp Med Biol,2000, 475 : 123.
  • 7Bjornheden T, Levin M, Evaldsson M. Evidence of hypoxic areas within the arterial wall in vivo[ J]. Arterioscler Thromb Vasc Biol. 1999,19 (4) ,870.
  • 8Shatrov VA, Sumbayev VV, Zhou J,et al. Oxidized low-density lipoprotein (oxLDL) triggers hypoxia-inducible factor-1 alpha (HIF-1 alpha ) accumulation via redox-dependent mechanisms [ J]. Blood ,2003,101 (12) :4847.
  • 9Jiang G, Li T, Qiu Y, et al. RNA interference for HIF-1α inhibits foam cells formation in vitro [ J ]. Eur J Pharm , 2007,562 (3) :183.
  • 10Ridker PM. Inflammatory biomarkers, statins, and the risk of stroke : cracking a clinical conundrum [ J ]. Circulation, 2002, 105(22) :2553.

二级参考文献22

  • 1杨胜利,刘惠亮,何作云.他汀类药物对血管及血栓形成的作用[J].心血管康复医学杂志,2005,14(6):589-592. 被引量：3
  • 2Neuhaus O,Strasser-Fuchs S,Fazekas F,et al.Statins as immunomodulators:comparison with interferon-beta 1b in MS[J].Neurology,2002,59:990-997.
  • 3Pruefer D,Makowski J,Schnell M,et al.Simvastatin inhibits inflammatory properties of staphylococcus aureus alpha-toxin[J].Circulation,2002,106:2104-2110.
  • 4Kawakami A,Tanaka A,Nakajima K,et al.Atorvastatin attenuates remnant lipoprotein-induced monocyte adhesion to vascular endothelium under flow conditions[J].Circ Res,2002,91:263.
  • 5Leitinger B,Hogg N.The involvement of lipid rafts in the regulation of integrin function[J].J Cell Sci,2002,115:963-972.
  • 6Lum AF,Green CE,Lee GR,et al.Dynamic regulation of LFA-1 activation and neutrophil arrest on intercellular adhesion molecule 1 (ICAM-1) in shear flow[J].J Biol Chem,2002,277:20660-20670.
  • 7Wierzbicki AS,Mikhailidis DP,Reynolds TM.More on PROSPER[J].Lancet,2003,361:1135-1136.
  • 8Leu HB,Chen JW,Wu TC,et al.Effects of fluvastatin,an HMG-CoA reductase inhibitor,on serum levels of interleukin-18 and matrix metalloproteinase-9 in patients with hypercholesterolemia[J].Clin Cardiol 2005,28(9):423-428.
  • 9Edwards CJ.Statins and bone morphogenetic proteins:new pathways in bone formation[J].Ann Acad Med Singap,2002,31:245-247.
  • 10Hogenboom S,Romeijn GJ,Houten SM,et al.Absence of functional peroxisomes does not lead to deficiency of enzymes involved in cholesterol biosynthesis[J].J Lipid Res,2002,43:90-98.

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药学实践杂志
2009年 第3期

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