芥子气促进真皮成纤维细胞分泌IL-10的时效和量效研究

Study on secretion of IL-10 stimulated by sulfur mustard in Human fibroblasts

目的:探讨芥子气诱导真皮成纤维细胞分泌IL-10的规律。方法:采用ELISA方法,检测不同浓度梯度的芥子气调节IL-10分泌的量效和时效关系。结果:成纤维细胞自身可分泌一定量的IL-10,芥子气作用成纤维细胞后,显著上调IL-10的分泌(P<0.05)。并呈时间依赖性和剂量依赖性。结论:芥子气治疗银屑病的作用机制可能与促进细胞分泌抗炎因子IL-10有关。

Objective：To explore the rule of secretion of IL-10 stimulated sulfur mustard in human fibroblasts. Methods： The ELISA was used to detect the relationship between dose and efficacy, time course and efficacy of which sulfur mustard adjusted the secretion of IL-10. Results： A portion of IL-10 could be secreted by the human fibroblasts. The secretion of IL-10 could be significantly stimulated after the function of sulfur mustard which depended on the time-course and dosage. Conclusion：The therapeutic mechanism of sulfur mustard to Psoriasis might related with the promotion of the secretion of IL-10.