^79Se-AMS生物示踪方法学的研究

Biological Tracer Methodology of ^(79)Se-AMS

由于具有放射性非常弱以及对生物组织甚至是细胞无损伤的特点,79Se是对硒元素进行长期无损伤生命科学研究的理想示踪核素7。9Se示踪方法学的建立对于硒的生命科学与生物医学研究将具有十分特殊的意义。加速器质谱测量79Se(79Se-AMS)的生物示踪方法学具有高灵敏度,高准确性以及高精度等特点。通过采用79Se-AMS方法对亚毒性剂量的硒代谢过程进行研究,建立了79Se-AMS生物示踪方法学,重点探索了生物示踪样品的制备流程,化学处理以及样品测量过程。采用分子负离子的引出形式以及四阳极双栅电离室对79Se和79Br两种同量异位素进行鉴别,从而记录实际样品中79Se的原子个数。实验建立了亚毒性剂量硒在Wistar大鼠肾脏内的代谢曲线,为生命科学以及生物医学领域提供了新的测量方法与手段,也为AMS的应用开拓了新的领域。

^ 79Se, with little radioactivity and little intrusiveness to biological tissue even to cell, is an ideal tracer nuclide for long period and non-intrusive biological research of selenium. Establishment of its tracer methodology is significant in biomedicine and life science. ^79Se-AMS is an alternative owing to its high sensitivity, accuracy and precision. Tracer experiment using ^79 Se was carried out to investigate the metabolism kinetics of selenium in the rat kidney, and therefore establishment of ^79Se-AMS biological tracer methodology is detailed, including tracing sample preparation, chemical procedure, and AMS measurement processing. During AMS measurement, molecular negative ions of AMS were extracted from the ion source, and the counts of ^79Se were determined from the discrimination between ^79 Se and ^79 Br by four-anode double-grid ionization chamber. Metabolism curve of subpoisonous selenium in the kidney of Wistar rat was established finally. Further applications of ^79Se-AMS for biological research will be explored.