COPD患者血清TNF-α、IL-8及MMP-9的检测及临床意义

Levels of Serum TNF-α, IL-8 and MMP-9 in COPD Patients and Its Clinical Significance

【目的】探讨慢性阻塞性肺疾病（COPD）患者血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）及基质金属蛋白酶-9（MMP-9）的含量变化及意义。【方法】抽取COPD急性期、缓解期患者及健康对照者静脉血，双抗夹心ELISA法检测血清中TNF-α、IL-8及MMP-9水平。【结果】C0PD急性期组TNF-α和IL-8水平显著高于缓解期及对照组；缓解期组IL-8水平显著高于对照组，急性期组及缓解期组MMP-9水平均显著高于对照组，以上差异均有显著性（P〈o．05）。【结论】TNF-α、IL-8及MMP-9参与了COPD的炎症过程，TNF-α及IL-8可在一定程度反映COPD患者病情变化及严重程度。

[Objective] To investigate the levels of serum TNF-α, IL-8 and MMP-9 in patients with chronic obstructive pulmonary disease（COPD） and to discuss the clinical significance. [Methods] Blood samples in patients with COPD both during acute exacerbation and stable stage were collected. Healthy volunteers were enrolled as controls. The levels of serum TNF-α, IL-8 and MMP-9 were measured by ELISA. [Results] The levels of TNF-α and IL-8 in patients with COPD during acute exacerbation were significantly higher than those in patients with COPD during stable stage and in the controls. The levels of IL-8 in patients with COPD during stable stage were significantly higher than those in the controls. The levels of MMP-9 in patients with COPD both during acute exacerbation and stable stage were significantly higher than those in the controls. [Conclusion]TNF-α, IL-8 and MMP-9 may take part in the inflammation activity of COPD. The levels of TNF-αand IL-8 may reflect the development and severity of COPD.