摘要
目的探讨非甾体类抗炎药尼美舒利对肺腺癌患者癌组织中环氧化酶2(COX-2)和血管内皮生长因子(VEGF)mRNA与蛋白表达的影响及其抗凋亡作用。方法选取手术治疗的肺腺癌患者60例,随机分为实验组(口服尼美舒利)(29例)和对照组(31例)。分别用RT-PCR、Western blot测定两组标本中COX-2和VEGFmRNA和蛋白的表达,用流式细胞仪检测其凋亡率。结果实验组COX-2和VEGFmRNA的表达较对照组减弱(P〈0.05),蛋白的表达也有同样的趋势;且实验组细胞凋亡率高于对照组,(13.47±1.54)%VS(4.26±0.87)%(P〈0.05)。结论术前服用尼美舒利可抑制肺腺癌患者肿瘤组织中COX-2和VEGF的表达,并可诱导其凋亡。
Objective To explore the effects of nimesulide, a selective cyclooxygenase-2 (COX-2) inhibitor,on the mRNA and protein expressions of COX-2 and VEGF and apoptosis in carcinoma tissue of lung adenocarcinoma. Methods Sixty patients with lung adenocarcinoma who underwent operation-therapy were randomly divided into study group (administered with nimesulide, 29 cases) and control group (31 cases). RT-PCR and Western blot were used to detect the mRNA and protein expressions of COX-2 and VEGF in lung adenocarcinoma tissue obtained from the patients. Flow cytometry was also performed to detect the apoptosis rate of adenocarcinoma cells. Results The mRNA expressions of COX-2 in the study group were lower than those in the control group. Meanwhile, a similar tendency developed to the protein expression of COX-2 and VEGF. Furthermore,the apoptosis rate was higher in the study group than that in the control group,(13.47±1.54)% vs (4.26±0.87)%( P〈0.05). Conclusions Nimesulide can inhibit the expression of COX-2 and VEGF in carcinoma tissue of lung adenocarcinoma,and induce the apoptosis of adenocarcinoma cells.
出处
《国际呼吸杂志》
2009年第12期705-708,共4页
International Journal of Respiration
基金
河北省卫生厅基金项目(07296)
关键词
肺腺癌
环氧化酶2
血管内皮生长因子
尼美舒利
人
Lung adenocarcinoma
Cyclooxygenase-2
Vascular endothelial growth factor
Nimesulide
Human