非甾体类抗炎药尼美舒利对肺腺癌的抑制作用

Inhibitory effects of nimesulide on human lung adenocarcinoma

目的探讨非甾体类抗炎药尼美舒利对肺腺癌患者癌组织中环氧化酶2（COX-2）和血管内皮生长因子（VEGF）mRNA与蛋白表达的影响及其抗凋亡作用。方法选取手术治疗的肺腺癌患者60例，随机分为实验组（口服尼美舒利）（29例）和对照组（31例）。分别用RT-PCR、Western blot测定两组标本中COX-2和VEGFmRNA和蛋白的表达，用流式细胞仪检测其凋亡率。结果实验组COX-2和VEGFmRNA的表达较对照组减弱（P〈0．05），蛋白的表达也有同样的趋势；且实验组细胞凋亡率高于对照组，（13．47±1．54）％VS（4．26±0．87）%（P〈0．05）。结论术前服用尼美舒利可抑制肺腺癌患者肿瘤组织中COX-2和VEGF的表达，并可诱导其凋亡。

Objective To explore the effects of nimesulide, a selective cyclooxygenase-2 （COX-2） inhibitor,on the mRNA and protein expressions of COX-2 and VEGF and apoptosis in carcinoma tissue of lung adenocarcinoma. Methods Sixty patients with lung adenocarcinoma who underwent operation-therapy were randomly divided into study group （administered with nimesulide, 29 cases） and control group （31 cases）. RT-PCR and Western blot were used to detect the mRNA and protein expressions of COX-2 and VEGF in lung adenocarcinoma tissue obtained from the patients. Flow cytometry was also performed to detect the apoptosis rate of adenocarcinoma cells. Results The mRNA expressions of COX-2 in the study group were lower than those in the control group. Meanwhile, a similar tendency developed to the protein expression of COX-2 and VEGF. Furthermore,the apoptosis rate was higher in the study group than that in the control group,（13.47±1.54）% vs （4.26±0.87）%（ P〈0.05）. Conclusions Nimesulide can inhibit the expression of COX-2 and VEGF in carcinoma tissue of lung adenocarcinoma,and induce the apoptosis of adenocarcinoma cells.