摘要
A RAPD marker specific to leaf shape of fig(Ficus carica)was developed and converted into SCAR marker for germplasm identification.RAPD amplifications with random primers S_ 2082 produced a unique fragment linking to the non-cordate base leaf shape.The unique fragment was extracted,cloned and sequenced.Sequencing results indicated that the size of the fragment was 1 640 bp.Specific primers were designed based on its sequence and used in SCAR-PCR,and it was showed that the RAPD marker from S_ 2082-1640 was successfully converted into SCAR marker.
A RAPD marker specific to leaf shape of fig （ Ficus carica ） was developed and converted into SCAR marker for germplasm identification. RAPD amplifications with random primers S2082 produced a unique fragment linking to the non-eordate base leaf shape. The unique fragment was extracted, cloned and sequenced. Sequencing results indicated that the size of the fragment was 1 640 bp. Specific primers were designed based on its sequence and used in SCAR-PCR, and it was showed that the RAPD marker from S2082- 1640 was successfully converted into SCAR marker.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2009年第6期158-161,共4页
Scientia Silvae Sinicae
基金
青岛农业大学校级重点课题(610607)
