摘要
本试验以草地早熟禾无菌种子苗茎尖为外植体,建立了高效的草地早熟禾丛生芽离体培养体系。结果表明:茎尖在附加0.5mg/L2,4-D和3.0mg/L6-BA的MS培养基上可诱导茎尖部位基部膨大,且诱导率最高。膨大的基部在2.0mg/L6-BA的丛生芽诱导培养基上,其诱导频率最大,然后将丛生芽转入附加3.0mg/L6-BA和0.07mg/L2,4-D的培养基上可进行继代培养,丛生芽在1/2MS培养基上生根率达100%。
In this research, an in vitro proliferation system of mutiple shoot clumps was established by using shoot apices of seedlings as explants at a high efficiency. The results showed that swelled bases were induced from shoot apices on MS medium supplemented with 0.5 mg/L 2, 4-D and 3.0 mg/L 6-BA, then were transferred to shoot clumps induction medium containing 2.0 mg/L 6-BA for the formation of mutiple shoot clumps, and multiple shoot clumps were obtained on proliferation medium supplemented with 3.0 mg/L 6-BA and 0.07 mg/L 2, 4-D. The rate of rooting of Poa pratensis L. on 1/2 MS medium reached 100 %.
出处
《草业与畜牧》
2009年第6期42-45,共4页
Pruataculture & Animal Husbandry
关键词
草地早熟禾
丛生芽
离体培养
Poa pratensis L.
Multiple shoot clumps
In vitro culture
