吡喹酮治疗前后小鼠脾细胞中CD4^+CD25^+Foxp3^+T细胞变化及其意义

Dynamics Changes of CD4^+CD25^+Foxp3^+ Regulatory T Cells in Schistosomiasis Mice

目的分析吡喹酮治疗前后小鼠脾细胞中CD4+CD25+Foxp3+T细胞的变化,探讨其与血吸虫感染后肝脏免疫病理反应的关系。方法54只BALB/C小鼠随机分为3组,阳性感染组、吡喹酮治疗组(感染后第6周顿服吡喹酮治疗)及阴性对照组。于感染后第3、6、9周分批处死小鼠,取脾单细胞悬液,经过常规表面染色、打孔、固定、胞内染色后,用流式细胞仪检测分析CD4+CD25+Foxp3+调节性T细胞的变化。结果感染组第3、6、9周脾细胞中CD4+CD25+Foxp3+T细胞的数量分别为(2.57±0.13)%、(1.77±0.21)%、(1.10±0.05)%,其含量逐步下降(P<0.05),治疗组分别为(2.61±0.12)%、(1.82±0.14)%、(1.76±0.11)%,经治疗后其含量变化差异无统计学意义(P>0.05),阴性组差异无统计学意义(P>0.05);CD4+CD25+Foxp3-T细胞和CD4+CD25-Foxp3+T细胞在各组中的含量差异均无统计学意义(P均>0.05)结论CD4+CD25+Foxp3+T细胞在日本血吸虫感染中具有免疫抑制作用,与血吸虫虫卵肉芽肿慢性病变具有相关性,吡喹酮可以通过抑制CD4+CD25+Foxp3+T降低以减缓血吸虫肉芽肿炎性病变的程度。

Objective To analyse the dymamic changes of CD4^＋ CD25^＋ Foxp3＋T cells before and after treatment of praziquantel in schistosomiasis mice and to explore the pathogenic reaction of mice ofter infected with schistosomiasis. Method 54 BALB/c mice were randomly derided into 3 groups i. e, praziquantel treated group, negative control group and positive control group, and the mice were killed at 3, 6, 9weeks after keing infected or freated. Then a spleen single cell suspension and a flow cytometry analysis of the CD4^ ＋ CD25^＋ Foxp3 ^＋ T cells were made. Result The results showed that the CD4^＋ CD25 ^＋ Foxp3^ ＋ T cells were declined remarkably from the 6 weeks after infection（P 〈0. 05）, but the CD4^＋ CD25^- Foxp3 ＋T cells and the CD4^＋ CD25 ^＋ Foxp3 - T cells had no change（P 〉 0. 05）. For the uninfected mice, all of the T ceils had no change（P 〉0. 05）. For the treatment mice, it had no change after treatment（P 〉0. 05）. Conclusion It suggested that it was the CD4^ ＋ CD25^ ＋ Foxp3^ ＋ T cells correlated closely to the murine schistosomiasis chronic hepatic fibrosis and the praziquantel could lighten the chronic inflammation of the liver through adjustment the CD4^ ＋ CD25^ ＋ Foxo3 ^＋ T cells.