小檗碱衍生物HB-13抗单纯疱疹病毒-2作用机制的研究

Research of antiviral mechanisms of action of berberine derivate HB-13 against herpes simplex virus type 2

目的探讨小檗碱衍生物HB-13抗单纯疱疹病毒-2(HSV-2)的作用机制。方法采用细胞病变法检测HB-13是否对HSV-2有直接作用;空斑法检测HB-13是否抑制HSV-2的吸附和释放;荧光定量PCR检测HB-13是否影响HSV-2 DNA复制以及蛋白免疫印迹方法检测HB-13是否影响HSV-2蛋白质合成。结果HB-13在细胞外无直接杀灭HSV-2作用;对HSV-2的吸附和释放亦无影响;不抑制HSV-2 DNA复制;但HB-13浓度依赖性地抑制HSV-2蛋白质合成。结论HB-13可抑制HSV-2蛋白质合成,其抗HSV-2作用机制与ACV等核苷类药物不同。

Objective To investigate the action mechanisms of berberine derivate HB-13 against herpes simplex virus type 2 （HSV-2）. Methods cytopathic effect （CPE） assay, plaque assay, FQ-PCR and Western blotting methods were applied to determine whether HB-13 had an effect of killing vii＇uses outside Vero cells and could affect the HSV adsorbtion, release, DNA replication and proteins synthesis. Results HB-13 had no effect on killing HSV-2 outside cells; and didn ＇t affect HSV-2 adsorbtion and release from Vero cells; and also didn＇t impact HSV-2 DNA replication, but it could inhibite HSV-2 protein synthesis in a concentration dependent behavior. Conclusions HB-13 could inhibit HSV-2 protein synthesis, which was different inhibition mod.e of action from that of acyclovir and ACV- related nucleoside analogs .