培养基成分对肠膜明串珠菌肠膜亚种合成葡聚糖的影响

Effect of medium composition on dextran producted by leuconostoc mesenteroides subsp. mesenteroides

通过单一因素法和正交法研究了分离自传统发酵乳中的肠膜明串珠菌肠膜亚种DM1-2-2生成葡聚糖的最佳培养基。葡聚糖合成量的测定采用苯酚-硫酸法。结果表明,酵母粉、20%的蔗糖、柠檬酸钠和醋酸钠不仅能显著提高葡聚糖的生物合成量,而且能明显促进菌体的生长。最佳的培养基组成:酵母粉20 g/L,蔗糖200 g/L,柠檬酸钠3 g/L,醋酸钠1 g/L时(均为质量浓度),葡聚糖的生物合成量为(4.21±0.15)g/L,产量较原来提高1.4倍。为葡聚糖的研究开发和生产应用提供了实验数据和理论依据。

The purpose was to study the optimal mediums composition for producing glucan by Leuconostoc mesenteroides subsp, mesenteroides DM1-2-2 isolated from traditional fermented milk through single factor and orthogonal experiments. The production of dextran was analyzed by phenol-sulphoacid method.The result shows yeast extract, sucrose, sodium citrate and sodium acetate could remarkably increase the production of dextran and encouraged the growth of strain evidently. The optimal mediums composition are： yeast extract 20 g/L, sucrose 200 g/L, sodium citrate 3 g/L and sodium acetate 1 g/L. The yield ofdextran are （4.21±0.15） g/L and improve 1.4 times under the optimal culture medium.