PTEN基因甲基化与胃癌临床病理特征的关系

The methylation of PTEN gene in gastric carcinoma

目的研究胃癌组织中抑癌基因第10号染色体缺失性磷酸酶张力蛋白基因（PTEN）表达异常与胃癌临床病理特征的关系。方法应用甲基化特异性PCR方法（MSP）检测45例胃癌及癌旁正常组织PTEN甲基化的表达情况。结果40．0％（18／45）的胃癌组织和2．2％（1／45）的癌旁正常组织PTEN基因发生甲基化，胃癌组织甲基化率显著增高（P〈0．05）；低分化腺癌甲基化率为60．0％（15／25），高中分化腺癌甲基化率为15．0％（3／20），二者差异具有统计学意义（P〈0．05）；发生淋巴结转移的24例胃癌组织中，13例PTEN基因发生甲基化，发生淋巴结转移的胃癌组织PTEN甲基化率明显高于无淋巴结转移胃癌组织（P〈0．05）。结论PTEN基因甲基化与胃癌的发生密切相关，PTEN基因甲基化在胃癌的发病过程中起重要作用。

Objective To explore the relationship between the abnormal expression of anti-oncogene PTEN in gastric carcinoma and the clinicopathological characteristics. Methods Mythylation specific PCR was applied to detect the expression of PTEN methylation in gastric carcinoma and their normal tissues from 45 patients. Results The methylation took place in 40.0 percent in gastric carcinoma and 2.2 percent in their normal tissues, and the difference was significant（P 〈0.05）. The methylation rate in poorly differentiated adenocarcinoma was 50.0 percent ,while 15.0 percent in highly-moderately differentiated group, the difference was significant （P 〈0.05）. In the 24 cases with lymph node metastasis, mythylation was observed in 13 cases, and the difference was significant （P 〈0.05）. Conclusion The methylation of PTEN gene was associated with gastric carcinoma, it may play an important role in the development of the disease.