摘要
目的探讨P—AKT异常表达与甲状腺滤泡癌血管生成之间的关系。方法采用免疫组织化学PV-6000两步法检测甲状腺滤泡癌、甲状腺腺瘤及结节性甲状腺肿标本中P—AKT、VEGF及CD34的表达。结果P—AKT在甲状腺滤泡癌中的表达较甲状腺腺瘤和结节性甲状腺肿中显著增高（P〈0．001）。甲状腺滤泡癌组织中，P—AKT、VEGF蛋白表达均与微血管密度（MVD）相关（P〈0．05），P—AKT阳性标本的MVD值高于P—AKT阴性标本（P〈0．05），VEGF阳性标本的MVD值亦高于VEGF阴性标本（P〈0．05）。P-AKT表达与VEGF表达的相关性有统计学意义（P〈0．05）。结论P-AKT在甲状腺滤泡癌中的高表达在甲状腺滤泡癌的发生发展及继发转移过程中发挥着重要作用。甲状腺滤泡癌中可能存在AKT／VEGF通路，AKT是调控VEGF表达通路的关键因子。
Objective To study the abnormal expression of P-AKT in follicular thyroid carcinoma and its relationship with angiogenesis. Methods MVD and expressions of P-AKT, VEGF in 40 specimens of FTC tissues were detected by immunohistochemical PV6000 method. Results The expression rate of P-AKT in FTC was higher than that of non-FTC tissues. The result was of statistical significance （P 〈0.001）. There were correlations between the MVD of FTC tissue and the expression of P-AKT and VEGF （P 〈0.05）. The MVD of P-AKT positive FTC tissue was higher than that of negative tissues （P 〈0.05）. The MVD of VEGF positive FTC tissue was also higher than that of negative tissues（P 〈0.05）. There were correlations between the expression of P-AKT and VEGF（P 〈0.05）. Conclusion The hyperexpression of P-AKT played an important role in the secondary deposits and development of human follicular thyroid carcinoma. AKT/VEGF pathway may exist in FTC, and AKT is the key factor in the pathway which regulated the expression of VEGF.
出处
《肿瘤研究与临床》
CAS
2009年第6期377-379,共3页
Cancer Research and Clinic
基金
山东省自然科学基金（Y2007C028）
