微量进样-火焰原子吸收光谱法测定小白鼠血液中镉

FAAS Determination of Cadmium in Blood of White Mouse with Micro Sample Introduction

离心分离小白鼠血液中的血红细胞和血清，所得的血红细胞经硝酸和过氧化氢消化处理。结合微量进样系统，进样量为300μL，用火焰原子吸收光谱法测定了小白鼠血液中血红细胞和血清中镉的含量。镉的质量浓度在0．050-0．600mg·L^-1范围内与吸光度呈线性关系，检出限（3s／k）为2．2μg·L^-1。加标回收率为97．1％，相对标准偏差（n=6）为3．0％。应用此法测定了饮用0．1g·L^-1硝酸镉溶液15d的小白鼠的血清和血红细胞中镉的含量，试验组的血液中镉含量为对照组的7倍。

The blood plasma and red blood cell in blood of white mouse were separated by centrifugation. The red blood cell was digested with HNO3 and H2O2. The content of CA in blood plasma and red blood cell was determined by FAAS with micro-sample introduction system of 300 μL. Linear relationship between values of absorbance and mass concentration of CA was obtained in the range of 0. 050--0. 600 mg · L-1 , with detection limit （3s/k） of 2. 2 μg · L^-1. Value of recovery by standard addition method was found to he 97. 1% with RSD＇s （n=6） of 3. 0%. The proposed method was used in the determination of Cd in blood plasma and red blood cell in blood samples of white mice which had been fed with 0. 1 g· L^-1 Cd（NO3）2 solution for 15 days, and the content of Cd of test group was 7 times higher than that of the control group.