毛细管电泳手性拆分喹诺酮类药物对映体

Chiral Separation of Enantiomers of Quinolone Drugs by Capillary Electrophoresis

采用毛细管电泳法,以铜(Ⅱ)-L-异白氨酸为手性拆分剂,同时分离了氧氟沙星、洛美沙星、司帕沙星和加替沙星四种喹诺酮类药物对映体。考察了手性拆分剂的种类、配比和浓度,缓冲溶液的种类、浓度和pH值,有机添加剂的种类和用量,分离电压等试验条件对分离效果的影响。含8 mmol.L-1L-异白氨酸和4 mmol.L-1硫酸铜的pH 8.5的30 mmol.L-1硼酸钠-盐酸缓冲溶液中,氧氟沙星和加替沙星对映体实现分离;在含20 mmol.L-1L-异白氨酸,10 mmol.L-1硫酸铜和乙腈(5+95)的pH 9.0的20 mmol.L-1的Tris-硼酸钠缓冲溶液中,司帕沙星、洛美沙星和加替沙星对映体同时实现完全分离。

Separation of enantiomers of 4 quinolones （ofloxacin, lomefloxacin, sparfloxacin, and gatifloxacin） were preformed simultaneously by capillary electrophoresis with copper（ Ⅱ ）-L-isoleucine as chiral resolving agent. Various factors influential to the efficiency of separation, including kinds of ehiral resolving agent, ratio and / concentration of chiral resolving agent, kinds of buffer solutions, pH and concentration of buffer solution, kinds and amount of organic additives, were studied and optimized. The enantiomers of ofloxacin and gatifloxacin were completely separated in 30 mmol·L^-1 Na2B4O7-HCl buffer solution of pH 8. 5 containing 8 mmolmmol·L^-1 L-isoleueine solution and 4 mmol·L^-1 CuSO4 solution, the enantiomers of omefloxacin, sparfloxaein, and gatifloxaein were completely separated in 20 mmol·L^-1 Tris-Na2B4O7 buffer solution of pH 9. 0 containing 20 mmol·L^-1 L-isoleucine solution, 10 mmol·L^-1 CuSO4 solution with addition of 5% by volume of acetonitrile.