摘要
在pH10.0的缓冲介质中,以235nm和360nm为发射波长测定时,邻菲口罗啉试剂的荧光因与镁(Ⅱ)反应生成了络合物而得到增敏,其增敏的程度(△F)与镁(Ⅱ)的质量浓度之间在0.1~2.0mg·L^-1范围内呈线性关系。此反应的检出限(3s/b)为0.038mg·L^-1。应用此方法测定了3种不同来源的水样中镁的含量,测定值的相对标准偏差(n=6)在1.3%~1.6%之间。在此样品的基础上用标准加入法做回收试验,测得回收率在98%~103%之间。
In a buffer medium of pH 10, fluorescence of the reagent o-phenanthroline was enhanced due to the formation of a complex with Mg^2+ ion, when measured at the wavelengths of 235 nm (λex) and 360 nm (λem). The magnitude of increase in fluorescence intensity (△F) was found to keep linear relationship with concentration of Mg^2+ ion in the range from 0. 1--2. 0 mg ·L^-1. Detection limit (3s/b) found for the method was 0. 038 mg ·L^-1. Three water samples of different sources were analyzed for their magnesium contents by this method, values of RSD's (n=6) found were in the range from 1.3%--1.6%. Test for recovery was made, giving values of recovery ranged from 98%--103%.
出处
《理化检验(化学分册)》
CAS
CSCD
北大核心
2009年第6期719-720,723,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
江苏盐城师范学院科研基金资助项目(06YCKL152)
关键词
荧光光度法
荧光增敏作用
镁(Ⅱ)
邻菲哆啉
Fluorophotometry
Fluorescence sensitization
Magnesium( Ⅱ ) ion
o-Phenanthroline