汽巴蓝功能微球对人血清白蛋白的吸附性能研究

Studies of human serum albumin adsorption on cibacron blue F3GA functional microspheres

通过分散共聚制得了聚苯乙烯接枝聚醋酸乙烯酯(PSt-g-PVAc)微球,粒径控制在500～700nm之间。在碱性条件下将PSt-g-PVAc微球表面的PVAc链醇解为聚乙烯醇(PVA),得到了PSt-g-PVA微球,提高了其在水中的分散稳定性。使汽巴蓝(CB)与PSt-g-PVA表面的羟基进行亲核反应,制得了CB作为配体的新型吸附剂汽巴蓝功能微球(CB微球),元素分析测得CB微球表面的CB最大含量为139.22μmol/g,探讨了其在不同人血清白蛋白(HSA)浓度、pH值和吸附时间下对HSA的吸附性能。当HSA浓度为1.0mg/mL、pH为5.14时,CB微球对HSA的最大吸附量为40.9mg/g,并分析了CB微球与HSA的相互作用机理。由NaSCN进行解吸试验,发现可将吸附在CB微球上的HSA解吸92.11%以上,且CB微球的重复使用性能良好。

Polystyrene grafted poly（vinyl acetate） （PSt-g-PVAe） microspheres were obtained by dispersion copolymerization of PVAc macromonomer with styrene. The diameter of PSt-g-PVAc microspheres was controlled in range of 500-700nm. Polystyrene grafted poly（vinyl alcohol） （PSt-g-PVA） microspheres were derived from alkaline hydrolysis of PVAc chains on surfaces of PSt-g-PVAc microspheres in the presence Gf NaOH. Dispersion stability of the PSt-g-PVA microspheres was increased in water. The CB functional microspheres were prepared via the nucleophilic reaction between PSt-g-PVA microspheres with Cibacron Blue F3GA （CB）. The biggest amount of CB on surfaces of the functional microspheres was 139.22μmol/g based on the analysis of an elemental analysis instrument. The effects of origination concentration of human serum albumin （HSA）, adsorption time and pH on adsorption amount of the CB functional microspheres were studied. The biggest absorption amount of CB functional microspheres was 40. 9mg/g for HSA under conditions of HSA concentration is 1.0 mg/ml and pH=5.14. The interaction mechanism of CB functional microspheres between HSA was further discussed. Desorption ratio of 92.11% was achieved by using an eluent of 0.5mol/L NaSCN （pH=8.0）. The CB functional PSt-g-PVA microsspheres could be reused without significant decreases in the adsorption capacities.