硫酸锌拮抗矽尘细胞毒作用的研究

Zinc Sulfate against Cytotoxicity of Silicious Dust on Mice in vitro

采刚体外大鼠红细胞溶血实验和小鼠腹腔巨噬细胞体外培养的方法,研究了硫酸锌与石英尘对大鼠红细咆、小鼠腹腔巨噬细胞的联合作用。结果表明,硫酸锌能明显拮抗体外石英尘耐细胞的毒性作用,表现为染尘后同一时间硫酸锌与石英尘联合作用组和单纯石英尘作用组相比,红细胞溶血百分率降低(P<0.05);巨噬细胞成活率明显提高(P<0.05);巨噬细胞胞浆内乳酸脱氢酶(LDH)漏出量降低(P<0.05)。推测其拮抗作用机理可能为Zn^(+2)与石英尘表面离解的硅烷醇基团相结合,阻隔了该丛团对细胞的作用,从而降低了石英尘的毒作用。

The combined effects of zinc sulfate with silicious dust on red blood cells and peritoneal macrophages were investigated in mice by using red blood cell hemolysis test and peritoneal macrophage culture in vitro. The results showed that in the group of combined zinc sulfate with silicious dust, the rate of red blood cell hemolysis and the release of LDH from macrophages into the culture medium were statistically decreased (P<0. 05), and the survival rate of the macrophages obviously increased (P<0. 05) as compared with those in the group of silicious dust alone, indicating that zinc sulfate could obviously antagonize the cyto-toxicity of silicious dust on mice in vitro. It was suggested that the mechanism of antagonism might be that Zn is bound to the hydrated silanol group on the surface of silicious dust to separate the cells from silanol group, and subsequently to decrease the cytotoxicity of silicious dust.