酶电极法测定食品中葡萄糖含量的研究

THE DETERMINATION OF GLUCOSE CONTENT IN FOOD BY ENZYME ELECTRODE

方法：选用固定化葡萄糖氧化酶与过氧化氢电极构成酶电极葡萄糖生物传感分析仪，每次进样量２５μｌ测定。结果：２０秒可测出样品中葡萄糖含量，在１０ｍｇ／Ｌ～１０００ｍｇ／Ｌ范围内具良好的线性关系，连续测定２０次的变异系数小于２％。

Glucose oxidase was immobilized at a hydrogen peroxide electrode and the enzyme electrode was used for the amperometric determination of glucose content in food. The linear range was 10-1000mg/L for glucose with response of 20 seconds. The coefficient of variation was less than 2%. This biosensor analyser can be used widely to determine glucose contents in various food samples.