P13K、AktmRNA在白血病中的表达及其临床意义

Expression and clinical application of the mRNA of PI3K and Akt mRNA in leukemia

目的分析成年人各型急性白血病（AL）和慢性粒细胞白血病（CML）中P13K及其下游信号分子AktmRNA的表达情况及其与白血病诊断、治疗及预后的关系。方法研究对象分为AL初治组63例、缓解组10例、复发组7例，CML组14例，对照组11例。用半定量反转录聚合酶链反应技术检测各组P13K、Akt的mRNA表达水平。结果P13K在AL初治组、复发组、CML组表达水平高于对照组，而且CML组高于AL缓解组；Akt在CML组表达高于AL缓解组、对照组，复发组高于对照组。AL初治组P13K、AktmRNA表达阳性者缓解率低，阴性者缓解率高。结论P13K／Akt信号转导通路在转录水平参与了白血病的发生、发展；该通路成分阳性表达的AL患者缓解率低。

Objective To study the expression of PI3K and Akt mRNA in acute leukemia（AL） and chronic myelocytic leukemia （CML） and try to find out a relationship between the prognostic significance of acute leukemia and the expression level of PI3K and Akt. Methods The samples were collected from 63 AL patients, 7 relapsed AL patients, 14 CML patients and 10 AL patients in complete remission（CR） patients, 11 samples of normal controls（NC）. The expression of PI3K, Akt mRNA were measured by semi-quantity reverse transcription polymerase chain reaction（RT-PCR）. Results The expression of PI3K mRNA in AL and relapse group were significantly higher than that in normal control. In CML, the PI3K mRNA expression level was higher than that in NC and CR. The similar results of the expression of Akt mRNA were observed in CML, NC and CR. At the same time, the expression level of Akt mRNA in relapse group was significantly higher than that in NC. The complete remission rate in PI3K （＋） was lower than that in PI3K （-）. Similar result was obtained in Akt （＋） and Akt （-）. Conclusion The PI3K/Akt pathway may contribute to the occurrence of leukemia. The patients with positive expression of the members of this pathway had a lower remission rate.