应用抗体芯片筛选上皮性卵巢癌患者血清的差异表达蛋白

Using protein chips to explore the differential expression of serum proteins in healthy women and OEC patients

目的通过检测健康妇女与上皮性卵巢癌患者血清间蛋白质组表达谱，筛选出差异表达蛋白质，分析异常表达蛋白质的生物学功能。方法选取11例上皮性卵巢癌患者术前血清混合样品及其配对健康妇女血清混合样品经Cy3和Cy5双色荧光标记后与同一型号的单克隆抗体芯片杂交。根据杂交荧光信号强度，计算内源标准化信噪比（INR），并以INR〉2．0或〈0．7为临界值，筛选健康妇女与卵巢癌患者术前血清间差异表达的蛋白群。结果发现27名健康妇女与卵巢癌患者术前血清间明显差异表达的蛋白质，含高表达蛋白16个和低表达蛋白11个。结论蛋白抗体芯片的研究样品可以是组织，血清等。筛选出的“关键蛋白群”，有助于认识卵巢癌发生的分子机理，寻找用于监测卵巢癌进展、判断疗效和预后的分子标志物以及新的治疗靶标。

Objective To explore the differentially expressed serum proteins in healthy women and OEC patients by using protein chips. Methods Sera of 11 cases of EOC and 11 matched healthy women were labeled with cy3 and cy5 fluorescent dyes and then were made to hybridized with the same protein antibody chips. The internally normalized ratio （INR） was calculated according to the fluorescent intensity of each protein spot. The value of INR 〉 2. 0 or 〈 0. 7 was considered as a cut-value to screen the differentially expressed serum proteins in EOC patients and healthy women. Results Twenty-seven differentially expressed serum proteins were found in healthy women and EOC patients, including 17 up-regulated and 10 down-regulated proteins in EOC patients. Conclusion Proteome-proteln antibody chip can be used to detect the differentially expressed serum proteins in the tissues. These target proteins will facilitate the study of diagnostic biomarkers of EOC and aid in monitoring the therapeutic effect of EOC.