Oct-4在胃癌组织中的表达及临床意义

Expression of Oct-4 in gastric carcinoma and its clinical significance

目的研究干细胞相关标志物Oct-4在胃癌组织中的表达及临床意义。方法收集1996年6月～2006年3月胃癌手术石蜡标本63例,免疫组化法检测组织中Oct-4表达,分别以58例癌旁正常组织和10例正常胃黏膜组织标本作为对照。回顾63例患者的临床病理学指标,并分析各项指标与Oct-4表达的关系。对63例中的50例患者进行为期8年的生存随访,Kaplan-Meier法和Log-rank检验分析Oct-4表达与患者生存的关系;构建Cox回归模型,评估Oct-4作为胃癌患者预后的独立影响因素的可能性。结果Oct-4在胃癌组织中阳性表达率为80.95%(51/63),显著高于癌旁正常组织的5.2%(3/58)和正常胃黏膜组织(0)(P<0.01)。Oct-4表达的阳性程度与胃癌的临床分期呈正相关(P<O.01)。Oct-4表达在不同分化程度胃癌组织及不同年龄和性别患者间比较,差异无统计学意义(P>0.05)。Oct-4表达阳性程度高的患者生存时间明显短于Oct-4表达阳性程度低的患者(P<0.05)。Oct-4表达水平与胃癌患者预后无明显相关性(RR 0.554,95%CI 0.209～1.466)。结论Oct-4在胃癌组织中表达的阳性程度与肿瘤临床分期及患者生存期有关,但不是胃癌患者预后的独立影响因素。

Objective To investigate the expression of Oct-4 as a stem cell marker in gastric carcinoma tissues and its clinical significance. Methods From June 1996 to March 2006, 63 paraffin samples of gastric carcinoma tissues were obtained, and the expression of Oct-4 was examined by immunohistochemical staining. Another 58 normal tissues adjacent to gastric carcinoma and 10 normal gastric mucosa tissues were served as controls. The clinicopathological data of 63 patients with gastric carcinoma were retrospectively analysed, and their relationship with the expression of Oct-4 was analysed. Fifty of these 63 patients were followed up for 8 years. Kaplan-Meier method and Log-rank test were employed to explore thecorrelation between Oct-4 expression and survival, and Cox Regression analysis was performed to evaluate the possibility of Oct-4 expression as an independent prognostic factor for gastric carcinoma. Results The positive expression rate of Oct-4 in gastric carcinoma tissues was 80.95% （51/63）, significantly higher than that of normal tissues adjacent to gastric carcinoma （5.2%, 3/58） and normal gastric mucosa tissues （0） （P 〈 0.01）. The expression of Oct-4 was positively correlated to the clinical stage of gastric carcinoma（P 〈 0.01）. There was no significant difference in the expression of Oct-4 among patients with different differentiation, age, and gender （ P 〉 0.05）. Patients with higher expression of Oct-4 had significantly shorter survival time （P 〈 0.05）. The expression of Oct-4 was not significantly correlated with the prognosis of gastric carcinoma （RR, 0. 554; 95% CI, 0. 209 - 1. 466）. Conclusion The expression of Oct-4 is correlated with the clinical stage and survival time of patients with gastric carcinoma, however, it is not an independent prognostic factor for gastric carcinoma.