摘要
目的:初步研究链霉菌HNS2-2(StreptomyceteHNS2-2)生物活性物质的理化性质。方法:以金黄色葡萄球菌(Staphlo-coccus)为指示菌,以抑菌圈直径为指标,测定链霉菌HNS2-2发酵液经不同理化因子处理后的抑菌效果。结果:该菌株发酵液中生物活性物质对80℃以下处理20min不敏感,40~80℃时抑菌圈直径平均为14.6mm,而100~120℃为10.2mm;pH 4~6时抑菌圈直径最大为15.2mm;阳光光照影响较大,照射2h其抑菌圈直径比日光灯照组减小31%;可用乙酸乙酯等极性较大的溶剂萃取,能溶于水;Doskochilov八溶剂系统纸层析属于多烯类抗菌活性物质。结论:该菌株发酵液中生物活性物质具有良好的热稳定性、耐酸碱性、水溶性和强极性,经纸层析初步鉴定为多烯类抗菌活性物质。
The research aimed to determin the physical- chemical character of Streptomycete HNS2 - 2 antimicrobial activity in fermentation broth. Method: Detemaination of influence of physical - chemical factors on antimicrobial activity antagonistic effect to Staphlococcus. Result: It was stable to the heat 40 - 80℃, pH 4 - 6 and natural light, but was unstable to sunshine and high consistency of acid and alkali. It is better to extracted with acetic ethe. Conclusion: Preliminary analysis on antibiotic property showed this is a kind of watersolubility, strong polarity products might be polyene.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第3期30-33,共4页
Biotechnology
基金
湖南省自然科学基金项目(08JJ6017)
湖南省教育厅科研项目(08C427)
国家自然科学基金面上项目(30771437)资助