链霉菌HNS2-2生物活性物质理化性质的初步研究

Preliminary Study on Physical-Chemical Character of Streptomycete HNS2-2 Antimicrobial Activity

目的:初步研究链霉菌HNS2-2(StreptomyceteHNS2-2)生物活性物质的理化性质。方法:以金黄色葡萄球菌(Staphlo-coccus)为指示菌,以抑菌圈直径为指标,测定链霉菌HNS2-2发酵液经不同理化因子处理后的抑菌效果。结果:该菌株发酵液中生物活性物质对80℃以下处理20min不敏感,40～80℃时抑菌圈直径平均为14.6mm,而100～120℃为10.2mm;pH 4～6时抑菌圈直径最大为15.2mm;阳光光照影响较大,照射2h其抑菌圈直径比日光灯照组减小31%;可用乙酸乙酯等极性较大的溶剂萃取,能溶于水;Doskochilov八溶剂系统纸层析属于多烯类抗菌活性物质。结论:该菌株发酵液中生物活性物质具有良好的热稳定性、耐酸碱性、水溶性和强极性,经纸层析初步鉴定为多烯类抗菌活性物质。

The research aimed to determin the physical- chemical character of Streptomycete HNS2 - 2 antimicrobial activity in fermentation broth. Method： Detemaination of influence of physical - chemical factors on antimicrobial activity antagonistic effect to Staphlococcus. Result： It was stable to the heat 40 - 80℃, pH 4 - 6 and natural light, but was unstable to sunshine and high consistency of acid and alkali. It is better to extracted with acetic ethe. Conclusion： Preliminary analysis on antibiotic property showed this is a kind of watersolubility, strong polarity products might be polyene.