LEPR基因Gln223Arg多态性与绝经后妇女骨质疏松症的相关性

Association between Gln223Arg polymorphism in leptin receptor gene and osteoporosis in postmenopausal women

目的探讨瘦素受体基因多态性与绝经后妇女骨密度（BMD）及骨质疏松症发生的关系。方法研究对象随机选择自2006年1月～2008年1月到骨科门诊就诊的绝经后妇女骨质疏松症患者,共200例;选择200例未发生骨质疏松症的老年绝经后女性作为对照组。测量各组成员股骨颈及腰椎骨的骨密度,并抽取外周血提取DNA并用PCR-RFLP方法检测各组成员的瘦素受体基因Gln223Arg基因型。结果绝经后妇女骨质疏松组与正常绝经后妇女对照组的Gln223Arg基因型频率分布有显著差异,绝经后妇女骨质疏松症患者中LEPR基因Gln223Arg各基因型之间腰椎骨的骨密度值有明显差异（P〈0.05）,而各基因型之间股骨颈的骨密度值无明显差异（P〉0.05）。结论LEPR基因Gln223Arg多态性与绝经后妇女骨质疏松症的发生及腰椎骨的骨密度值密切相关,与股骨颈的骨密度值无明显相关。

Objective To study the relationship between the LEPR polymorphism and the incidence of osteoporosis and BMD of postmenopausal women. Methods 200 postmenopausal women patients with osteoporosis who came to our hospital during 2006.1-2008.1 were selected, and selected 200 postmenopausal women without osteoporosis as control group. Measure the BMD of collum femoris and lumbar vertebra, and examine the genotype of LEPR Gln223Arg by the PCR-RFLP method. Results The difference of LEPR genotype between the experiment group and control group is significant （ P 〈 0. 05）. And the BMD of lumbar vertebra and the genotype of LEPR gene have the significant relationship（ P 〈 0.05）. The BMD of collum femoris between the 3 genotype have no significant differenee（P 〉 0.05）. Conclusion The polymorpbism of LEPR Gln223Arg is associated closely with the incidence of osteoporosis and the BMD of lumbar vertebra, have no relationship with the BMD of collum femoris.