运动与碳酸钙联合应用对去卵巢大鼠骨元素含量的变化

Effect of exercise and calcium carbonate on bone minerial element in ovariectomized rats

目的用测定骨元素含量的方法,观察运动和碳酸钙（CaCO3）联合应用对去卵巢大鼠骨元素代谢的影响。方法将健康4月龄雌性SD大鼠48只,按体重随机分成6组：正常对照组;假手术组;去卵巢组;去卵巢＋CaCO3组;去卵巢＋运动组;去卵巢＋CaCO3＋运动组。去卵巢＋CaCO3组和去卵巢＋CaCO3＋运动组。大鼠于去卵巢手术后第2 d开始给予CaCO3灌胃[元素钙20 mg/（kg.d）],持续11w。去卵巢＋运动组和去卵巢＋CaCO3＋运动组大鼠于去卵巢术后第7 d开始给予中等强度的运动训练,每周5 d,每天连续匀速跑45 min,16 m/min,跑道倾角0°,持续10w。结果去卵巢组大鼠骨Ca、S、Mg、Zn、Co、Mn等元素含量均明显低于假去卵巢组（P〈0.01）,骨P含量明显高于假去卵巢组（P〈0.01）;去卵巢＋CaCO3组和去卵巢＋运动组大鼠骨Ca、S、Mg、Zn、Co、Mn等元素含量虽然较去卵巢组明显增加（P〈0.01）,但仍显著低于假去卵巢组（P〈0.01）;运动与CaCO3联合应用后,去卵巢大鼠骨Ca、S、Mg、Zn、Co、Mn等元素含量均显著回升（P〈0.01）,骨P含量显著回降（P〈0.01）,并且基本恢复到对照组水平。结论运动可加强CaCO3对抗由于去卵巢引起的大鼠骨元素代谢紊乱的作用。

Objective To investigate the influence of calcium carbonate （ CaCO3 ） and exercise on bone mineral element of bilaterally ovariectomized rats. Methods 48 female Sprague-Dawley （SD）rats were divided randomly into six groups ： （ 1 ） Normal control group, （2） Sham-ovariectomized group, （ 3 ） Ovariectomized group （ OVX ）, （ 4 ） Ovariectomy plus CaCO3 group, （5） Ovariectomy plus exercise group, （6） Ovariectomy plus CaCO3 plus exercise group. The rats in the ovariectomy plus CaCO3 group and ovariectomy plus CaCO3 plus exercise group took CaCO3 （element calcium 20 mg/kg per day）from the 2nd day after operation for 11 weeks. The rats in the ovariectomy plus exercise group and ovariectomy plus CaCO3 plus exercise group received exercise training from the 7th day after operation,5 days a week for 10 weeks,45 minutes per day, 16 m per minute, the dip of the runway was 0°. Results （1）Ca, S, Mg, Zn, Co, Mn reduce and P increases in the bone of the OVX rats（ P 〈 0.01 ） ; （2） CaCO3 or exercise opposes the changes of Ca,P,S,Mg,Zn,Co, Mn in the bone of the OVX rats（P 〈 0.01 ） ; （3） Exercise strengthens the effect of CaCO3 （ P 〈 0. 01 ）. Conclusion Exercise strengthens the effect of CaCO3 on the changes of Ca, S, Mg, Zn, Co, Mn and P in the bone of the OVX rats.