血管紧张素原基因T704C多态性与脑血管病的关系

Relationship between polymorphism of angiotensinogen gene T704C and cerebrovascular disease

目的探讨血管紧张素原(AGT)基因T704C多态性与脑血管病(CVD)的关系。方法应用聚合酶链式反应-限制性片断长度多态性(PCR-RFLP)技术检测82例脑梗死患者(CI组)、70例脑出血患者(ICH组)和89名正常对照者(正常对照组)AGTT704C基因型和等位基因;分析和比较其在各组间分布的差异。Logistic回归分析AGTT704C基因型和脑卒中危险因素对CI、ICH发病的影响。结果AGT704CC基因型及C等位基因频率CI组(63.4%,79.9%)显著高于正常对照组(34.8%,61.2%)(均P<0.05);ICH组(41.4%,67.1%)与正常对照组比较差异无统计学意义。CI及ICH组中,有或无高血压病患者间CC基因型和C等位基因频率差异无统计学意义。Logistic回归分析显示:AGT704CC基因型和高血压对CI发病的相对危险度(OR)分别为3.667和6.709(均P<0.005);两者同时存在时发生CI的OR为30.295(P<0.005)。AGT704CC基因型不增加ICH发病概率。结论AGT T704C基因多态性可能是CI发病的遗传因素;AGT704CC基因型和高血压对CI发生具有协同效应;AGTT704C基因多态性可能与ICH发病无关。

Objective To investigate the relationship between polymorphisms of angiotensinogen （AGT） gene T704C and cerebrovascular disease（CVD）. Methods AGT T704C genotype and allele were examined by PCR-RLFP in 82 patients with cerebral infarction （ CI group）, 70 patients with intracerebral hemorrhage （ ICH group） and 89 age-matched normal controls（ NC group）. The AGT T704C genotype and allele frequencies among the 3 groups were compared and analyzed. The effeetes of AGT T704C genotype and the risk factors of stroke to induce CI snd ICH were analyzed by Logistic regression. Results The frequencies of AGT 704CC genotype and C allelic in CI group （63.4%, 79.9% ） were significantly higher than those in NC group（34.8% ,61.2% ） （ all P 〈 0. 05 ）, but there was no significantly difference between ICH group（41.4%, 67.1% ） and NC group. In CI group and ICH group, there was no significantly difference in frequence of CC genotype and C allele between the patients with or without hypertension. Logistic regression analysis showed that the OR of CI with AGT 704CC genetype and hypertension were 3. 667 and 6. 709, respectively （ all P 〈 0. 005 ） ; and with both of them was 30. 295 （ P 〈 0. 005 ）. AGT 704CC genotype was not increased the probabiluity of ICH. Conclusions The polymorphism of AGT T704C may be the genetic factor to incidence of CI. Hypertension and AGT 704CC genotype may have a coordinating function in the CI pathogenesis. The polymorphism of AGT T704C may be not related to the incidence of ICH.