猕猴桃果汁抗环磷酰胺致突变作用及其机制

THE STUDY ON ANTIMUTAGENIC MECHANISM OF KIWIFRUIT JUICE

目的：研究猕猴桃果汁抗环磷酰胺（ＣＰ）的致突变作用及其可能的作用机制。方法：测定大鼠外周血双核淋巴细胞微核细胞率及肝中超氧化物歧化酶（ＳＯＤ）活性，还原型谷光甘肽（ＧＳＨ）、丙二醛（ＭＤＡ）含量。结果：猕猴桃果汁对ＣＰ诱发的大鼠外周血双核淋巴细胞微核细胞率有显著抑制作用，能明显诱导大鼠肝ＳＯＤ活性增强，以及ＧＳＨ的含量增加，降低ＭＤＡ含量。大鼠外周血双核淋巴细胞微核细胞率与ＳＯＤ活性及ＧＳＨ含量呈明显负相关，与ＭＤＡ含量呈明显正相关。结论：猕猴桃果汁抗ＣＰ致微核形成作用的机制可能与通过提高机体抗氧化防御系统的功能，防止有害自由基对细胞内生物大分子的损伤有关。

Objective: To observe the effect of kiwifruit juice on the mutagenicity induced by cyclophosphamide (CP) and the antioxidative mechanism in rats. Methods: Measure the frequency of cells with micronuclei (MN) in binucleated lymphocytes and the activity of superoxide dismulases (SOD), the levels of glutathione (GSH) and malondialdehyde (MDA) in liver of rats Results: (1) Kiwifruit juice could inhibit the frequency of cytokinesisblocked cells with micronuclei induced by CP in rat peripheral blood lymphocytes. (2)Increased significantly the activity of liver SOD and the level of GSH and decreased the level of malondialdehyde. (3) The rate of MN cells had significant correlation negatively with the activity of SOD and GSH and positively with the level of MDA. Conclusion: The kiwifruit juice may promote the antioxidative functions and protect the biological macromolecules from the attack of free radicals. The mechanisms of its antimicronucleus formation may lie in this.