草苁蓉甲醇提取物对二乙基亚硝胺诱发大鼠肝脏癌前病变的抑制作用

Inhibitory Effect of Methanol Extract of Boschniakia rossica Fedtsch.et Flerov on Rat Hepatic Preneoplastic Lesions Induced by Diethylnitrosamine

目的：探讨草苁蓉（ＢＲ）对大鼠肝脏化学致癌作用的抑制效应。方法：按ＳｏｌｔＦａｒｂｅｒ氏方法复制大鼠肝脏癌前病变模型，用免疫组织化学方法观察了在肝脏癌前病变组和ＢＲ处理组及正常对照组大鼠肝脏中胎盘型谷胱甘肽Ｓ转移酶（ＧＳＴＰ），突变型Ｐ５３蛋白及Ｐ２１蛋白的表达水平。结果：发现５００ｍｇ／ｋｇ剂量的ＢＲ甲醇提取物对二乙基亚硝胺（ＤＥＮ）诱发Ｆ３４４大鼠肝脏ＧＳＴＰ阳性灶的形成有抑制作用，而且其突变型Ｐ５３蛋白及ｒａｓ基因产物ｐ２１蛋白的表达水平显著低于癌前病变组，并在ＢＲ处理后，在肝脏化学致癌初期由于癌前病变的形成所增高的γＧＴ活力明显下降。结论：提示ＢＲ对ＤＥＮ诱发大鼠肝脏癌前病变有抑制作用。

Objective: To investigate the inhibitory effect of Boschniakia rossica(BR)on hepatocarcinogenesis in rats. Method:Based on immunohistochemistry techniques,the expression of placental form glutathione Stransferase(GSTP),mutant p53 and p21 protein were investigated in hepatic preneoplastic lesions induced by SoltFarber protocol in the liver of rats that had been treated with the above method, administered with BR extract and of control groupResult:The extract of BR(500mg/kg)has inhibitory effect on the formation of diethylnitrosamineinduced GSTPpositive foci in F344 rat and the expression of mutant p53 and p21 protein was lower than that of hepatic preneoplastic lesions, and the increasing glutamyltranspeptidase(GT)activity in rat liver treated with SoltFarber protocol was decreased by the extract of BR,Conclusion:These results indicate that BR has inhibitory effect on DEN induced hepatic preneoplastic lesions in F344 rat