摘要
目的初步研究结肠切除对青紫兰兔结肠黏膜中5-羟色胺(5-hydroxy tryptamine,5-HT)及嗜铬粒蛋白A(chromogranin A,CgA)阳性细胞数量变化的影响。方法选取15只青紫兰兔,切取结直肠交界上段结肠7～8cm长(对照组),继续饲养2周后处死动物,取吻合口处结肠段(研究组)。免疫组化法检测2组结肠黏膜中的5-HT阳性细胞及CgA阳性细胞,观察其数量的变化。结果对照组与研究组结肠黏膜中的5-HT阳性细胞数分别为(10.40±2.22)个及(26.27±2.35)个,CgA阳性细胞数分别为(20.60±5.34)个及(51.51±6.13)个,差异均有统计学意义(P<0.01)。结论结肠切除可引起兔结肠黏膜中5-HT及CgA阳性细胞数量的增多。
Objective To investigate the influence of colectomy on the expressions of 5-hydroxy tryptamine （5-HT） and chromogranin A （CgA） in colon mucosa of Chinchilla rabbits. Methods Colon （7--8 cm） upon colonrectum junction （control group） of 15 Chinchilla rabbits was cut out. After two weeks, these rabbits were executed and the samples of colon at anastomotic stoma （study group） were taken. 5-HT positive cells and CgA positive cells in two groups were detected by immunohistoehemical method. Results The number of 5-HT positive cells was 10.40±2.22 in control group, and 26.27±2.35 in study group; the number of CgA positive cells was 20.60±5.34 in control group, and 51.51± 6.13 in study group. There were significant differences between two groups respectively （P〈0.01）. Conclusion The increase of 5-HT positive cells and CgA positive cells can be caused by colectomy.
出处
《中国普外基础与临床杂志》
CAS
2009年第6期452-454,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
福建省自然科学基金项目(项目编号:C0410044)~~
