胸腺素β10在肝细胞癌中的表达及其临床意义

Expression of thymosin beta-10 gene in hepatocellular carcinoma and its clinical significance

目的探讨人肝细胞癌(hepatocellular carcinoma,HCC)患者组织胸腺素β10(Thymosin beta-10,Tβ10)mRNA的表达及其临床意义。方法采用逆转录聚合酶链反应(RT-PCR)检测70例HCC患者癌组织、癌旁肝组织及18例正常肝组织中Tβ10 mRNA的表达情况,分析Tβ10 mRNA表达水平与肝癌患者的临床分期、有无术后复发、有无肝外转移、有无门静脉癌栓、肿瘤个数、肿瘤直径、血清AFP水平和病理分级等指标的关系。结果Tβ10 mRNA在正常肝组织(1.30±0.53)及癌旁肝组织(1.21±0.50)中的表达水平明显高于在HCC组织(1.01±0.41)中的表达水平(P<0.05),而在正常肝组织及癌旁肝组织中的表达水平差异无统计学意义(P>0.05)。Tβ10 mRNA在人HCC组织中的表达与门静脉癌栓、肿瘤个数及肿瘤直径明显相关(P<0.05),而与临床分期、肝外转移及术后复发、血清AFP水平及分化程度无明显关系(P>0.05)。结论Tβ10 mRNA在HCC组织中低表达,可能与HCC的发生、发展的早期阶段有关。

Objective To explore the expression of thymosin beta-10 mRNA in human HCC tissues and its clinical significance. Methods Reverse transcription polymerase chain reactions （RT-PCR） was employed to determine the expression of thymosin beta-10 mRNA in 70 cases of HCC and the adjacent liver tissues,as well as in 18 normal liver tissues. Results The expression level of thymosin beta-10 mRNA in normal liver tissue （1.30 ±0. 53） and adjacent liver tissue （1.21 ±0.50） was markedly higher than that in HCC tissues （ 1.01 ±0.41 ,P 〈0. 05）. The expression level of thymosin beta-10 mRNA were not significantly different between normal liver tissue and adjacent liver tissue （ P 〉 0. 05 ）. The expression of thymosin beta-10 in HCC tissue was significantly correlated with the portal vein tumor thrombus, the number and the size of tumor, but not correlated with the clinical stage of the patients, the presence of extrahepatic metastasis, the recurrence of tumor, the level of serum alphafetoprotein （AFP）, and the differentiation of tumor. Conclusion Thymosin beta-10 may play a role in initiation and progression of HCC.