实验性脾虚证大鼠胃窦部SP、VIP、CGRP含量的研究

An Investigation of SP,VIP,CGRP Levels in Gastric Autrum of Experimental Spleen Deficiency Rats

采用放射免疫分析方法（ＲＩＡ）观察了实验性脾虚证大鼠胃窦部Ｐ物质（ＳＰ）、血管活性肠肽（ＶＩＰ）、降钙素基因相关肽（ＣＧＲＰ）含量的变化。结果：脾虚组胃窦部ＳＰ、ＶＩＰ、ＣＧＲＰ免疫活性肽（ｉｒＳＰ、ｉｒＶＩＰ、ｉｒＣＧＲＰ）含量均明显降低，下降百分率为ｉｒＳＰ＞ｉｒＶＩＰ＞ｉｒＣＧＲＰ。自然恢复组ｉｒＳＰ含量无明显恢复，ｉｒＶＩＰ、ｉｒＣＧＲＰ含量均恢复正常。加味四君子汤治疗组ｉｒＳＰ含量有一定程度恢复，但与对照组相比仍有显著差异，ｉｒＶＩＰ、ｉｒＣＧＲＰ含量均恢复正常。结果提示：脾虚大鼠胃窦部ｉｒＳＰ、ｉｒＶＩＰ、ｉｒＣＧＲＰ含量的改变可能与脾虚证胃动力失调有一定关系。

ubstance P (SP),vasoactive intestinal peptide (VIP), calcitonin gene related peptide(CGRP) levels in all layers of gastric antrum in experimental spleen deficiency (SD) rats were measured by radioimmunoassys(RIA).The results show that: The ir SP,ir VIP,ir CGRP levels in gastric antrum of SD rats were significantly lower than that of control. The reduced percent is ir SP>ir VIP>ir CGRP. In spontaneous recovery cases, ir SP level was still less than control, while ir VIP, ir CGRP levels recovered. After treated with Sijunzi Tang, ir SP was slightly improved but still showed significantly difference compared with control. ir VIP, ir CGRP were improved to the levels of control group. Changes of SP,VIP, CGRP levels in gastric antrum of SD rats may be closely related to the gastrointestinal motility disorders presented in SD. The Chinese herbs(Sijunzi Tang) currently used have partially therapeutic effect.