新生大鼠离体脑干脊髓标本的制备及在呼吸中枢研究中应用

Preparation of the In Vitro Brainstem-Spinal Cord of theNewborn Rat and Its Use in Studies on Respiratory Center

本研究用０～４ｄ的Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ新生大鼠制备了离体脑干－脊髓标本，在浸浴并人工灌流改良Ｋｒｅｂｓ液的条件下，标本可存活７．５ｈ以上；在此标本上记录到了颈４，５脊神经腹根及延髓神经元的节律性放电活动。

The in vitro brainstem-spinal cord was prepred from 0～4 days old Sprague-Dawley rats. Placed in a bath and perfused with modified Krebs solution,the preparation was viable for more than 7.5 hours.The periodic discharge activity was recorded from ventral root c4 or c5 and the neurons of medulla of the preparation.The results indicate that in vitro brainstem-spinal cord preparation has powerful advantages in studies of respiratory center.