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随机引物多态性DNA及其在微生物分型中的应用 被引量:4

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摘要 用于微生物分型的现代技术包括表型和基因型方法。随机扩增多态性DNA(RandomAmplifictedPolymorphicDNA简称RAPD)是在PCR技术基础上发展起来的一种新的基因分型法。主要特点是利用随机寡核苷酸引物扩增基因组DNA片段,进而通过凝胶电泳分析其指纹图特征借以显示不同菌株间存在细微差别。这种方法简单、快速,现已在许多微生物分型中得到运用。
作者 王苏建
出处 《国外医学(临床生物化学与检验学分册)》 1998年第3期118-120,共3页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
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  • 1蔡子微,文姝,康白.对两株双歧杆菌耐药性及其影响因素的研究(摘要)[J].中国微生态学杂志,1994,6(5):59-59. 被引量:2
  • 2郑跃杰,潘令嘉,叶桂安,周殿元.次最低抑菌浓度抗生素对双歧杆菌粘附的影响[J].中国微生态学杂志,1996,8(5):4-6. 被引量:4
  • 3KAUFMANN P,FEFFERKORN A P,TEUBER M, et al. Identification and quantification of Bifidobacteria species isolated from food with genus specific16S rRNA-targeted probes by colony hybridization and PCR [J]. Appl Environ Microbiol, 1997,63 (3) : 1258-1273.
  • 4AKOPYANZ N,BUKANOY N O, WESLFTBLOM I V, et al. DNA diversity among clinical isolates of Helicobacter pylori defected by PCR based RAPD fingerprinting [ J]. Nucleic Acids Res, 1992,20 ( 5 ) : 5137-5142.
  • 5H'BYEON J H, YU S. Salmonella enterica serovar typhistains isolated in korea containing a muhidrug resistance class I integron[ J]. Antimicrob Agents Chemother,2003,47 (6) :2006-2008.
  • 6GAZEW H, ABDOUSLAM N, HAWKEY P M,et al. Incidence of class 1 integrons in squatemary ammonium compound-polluted enviroment[J]. Antimicrob Agents Chemother,2005,49 (5) :1802-1807.
  • 7TOIT M D U. Characterization and selection of probiotic lactobacilia for a preliminary minippig feeging trial and their effect on serum cholesterol levels, faeces pH and faeces moisture content [ J ]. Intern J Food Microbiol, 1998,40 ( 2 ) :93 -104.
  • 8BRUCKW M ,GRAVERHOLT G, GIBSON G R. A two-stage continuous culture system to study the effect of supple mental alpha-lactalbumin and glyeomacropeptide on mixed cultures of human gut bacteria challenged with enteropathogenic Escherichia coli and Salmonella serotype Typhimurium[j]. j Appl Microbiol,2003,95 ( 1 ) : 44-53.
  • 9DIETER K L. Quantification using real-time PCR technology : applications and limitations [ J ]. Trends Mol Med, 2002,8 ( 4 ) : 257-260.
  • 10YOUNG V B, SCHMIDT T M. Overview of the gastrointestinal microbiota[ J]. Adv Exp Med Bio,l 2008,635(1 ) :29-40.

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