前列腺素在紫外线上调纤溶活性中的作用

Role of Prostaglandins in Upregulation of Fibrinolytic Activity by Ultraviolet Irradiation

目的探讨前列腺素（ＰＧｓ）在紫外线（ＵＶ）上调血纤溶活性中的作用。方法１５只健康成年西北杂种犬被随机分成三组，ＵＶ组和ＵＶ＋阿斯匹林组行全光谱ＵＶ２０ｃｍ×２０ｃｍ、４４７．７２ｍＪ／ｃｍ２照射胸腹部１次，其中ＵＶ＋阿斯匹林组ＵＶ照射前在辐射区皮肤先进行直流电阿斯匹林离子导入１次；空白组既不照射ＵＶ也不行离子导入，作对照用。对比观察ＵＶ照射后４８小时内辐射区皮肤抽吸水疱液中ＰＧＥ２、ＰＧＤ２、６ｏｘｏＰＧＦ１α浓度及血纤溶酶原激活物（ＰＡ）、纤溶酶原激活抑制物活性的动态变化。结果ＵＶ组在ＵＶ照后３小时ＰＡ活性明显增高，照后９小时达峰值；辐射区皮肤抽吸水疱液中三种ＰＧｓ水平均在ＵＶ照后５小时明显升高，峰值约在ＵＶ照后５～９小时之间，三种ＰＧｓ水平变化与ＰＡ活性变化均无显著相关性；ＵＶ＋阿斯匹林组辐射区皮肤组织内三种ＰＧｓ水平无明显升高，ＰＡ变化规律与ＵＶ组相类似，两组间ＰＡ活性无显著性差异（Ｐ＞０．０５）。结论ＰＧＥ２、ＰＧＤ２。

Objective To elucidate possible role of prostaglandins in upregulation by ultraviolet(UV) irradiation on fibrinolytic activity.Methods Fifteen healthy adult northwest hybrid dogs were randomly divided into three groups,each with 5 dogs.UV and UV plus aspirin group were treated by single 20 cm×20 cm, 447.72 mj/cm 2 full spectrum UV irradiation on thoracoabdominal skins.Before irradiation,UV plus aspirin group accepted 300 mg aspirine by direct current ion introduction on the selected skins of irradiation.The control group got neither UV nor ion introduction of aspirin.In 48 hours,concentrations of PGE 2,PGD 2 and 6 oxo PGF 1α in the suction blister fluid of irradiated skins were assayed dynamically,and activities of plasminogen activators(PA) with their inhibitory substances were observed.Results In UV group,PA significantly increased after 3 hours of irradiation with peak at 9 hours.Levels of the three PGs in the suction blisters obviously elevated at 5 hours of irradiation with peaks in 5 to 9 hours.Between levels of the three PGs and PA activities,there were no significant correlations.However,in UV plus aspirin group the three PGs showed no significant changes and PA shared the same features of UV group (P> 0.05 ).Conclusion PGE 2,PGD 2,6 oxo PGF 1α may not participate in upregulation of fibrinolytic activity by full spectrum UV irradiation.