抑胃多肽主动免疫治疗对大鼠行为活动的影响

Effects of active immunization against gastric inhibitory popeptide (GIP) on behavior in rats

目的:观察抑胃多肽(GIP)靶向的主动免疫干预治疗对大鼠行为学的影响。方法:高脂喂养的SD大鼠分为实验组及对照组。实验组如期皮下多点注射GIP靶向的人工抗原(GIP-KLH);对照组如期皮下多点注射KLH。检测血清中GIP抗体滴度水平,观察疫苗诱生抗体对GIP促进胰岛β细胞MIN6细胞分泌胰岛素的影响及两组在行为学方面存在的差异。结果:实验组首次免疫接种后4周,即产生了高滴度GIP特异性抗体,如期继续免疫接种,抗体水平进一步增高;实验组血清较对照组可以显著抑制GIP的促MIN6细胞胰岛素分泌作用(P<0.01);行为学实验中,自发活动测试实验组总路程(P<0.01)、平均速度(P<0.01)、活动时间(P<0.05)、活动次数(P<0.01)均明显低于对照组,休息时间明显高于对照组(P<0.05),有统计学差异,Morris水迷宫测试两组无明显统计学差异(P>0.05)。结论:人工抗原GIP-KLH能打破机体免疫耐受,诱生GIP特异性抗体;GIP靶向的主动免疫干预治疗使大鼠自发活动性下降。

Objective：To investigate the effect of active immunization against gastric inhibitory popeptide （GIP）on behavior of SD rats. Methods：Male rats were fed with high-fat diet and divided into control group and GIP immunized group. Animals of experimental group and control group were immunized subcutaneously with 400 μg of GIP-KLH and KLH at the indicated time points respectively. GIP-specific antibody titers were determined by ELISA according to standard protocols differences on GIP-induced the insulin secretion of MIN6 cells using GIP-specific antibodies and behavior between two groups were investigated. Results：After four weeks of single immunization,high GIP-specific antibody titers were induced. Antibody levels further increased with continue injections. Compared with the control group, GIP-KLH immunized rats＇serum containing specific antibodies significantly inhibited GIP-induced the insulin secretion on MIN6 cells （P 〈 0.01 ）.Compared with control rats, distance （P 〈 0.01）, speed （P 〈 0.01 ）, active times （P 〈 0.05）, the number of activities（P 〈 0.01 ）in spontaneous activity test were different（P 〈 0.05）significantly in GIP immunised group. Both the two groups showed no significant differences in test section of Morris water maze test（P 〉 0.05）. Conclusion：Active immunization against GIP resulted in high, GIP-specific antibody titers. GIP immunized rats had a decreasing spontaneous activity.