江苏省2006年新发现感染者的HIV-1分子流行病学研究

Molecular epidemiology of the human immunodeficiency virus-1 isolated from patients confirmed lately in Jiangsu province

目的:了解人类免疫缺陷病毒在江苏省的亚型分布特点,初步探讨传播途径与病毒亚型间的关系。方法:收集2006年新发现HIV-1者的流行病学资料;用EDTA-K3抗凝的全血,提取病毒RNA,用逆转录巢式PCR扩增env基因的C2-V3区和gag基因。先用ClustalX对所得序列和参考株序列进行比对,然后用MEGA3.1构建进化树和亚型分析。结果:江苏省共发现CRF01_AE、CRF07_BC、CRF08_BC、B、C、CRF02_AG、A17种亚型,其中主要流行的亚型是CRF01_AE,占31.6%(24/76);其次为CRF07_BC,占25%(19/76);B亚型占19.7%(15/76)。在异性性传播途径中,共发现CRF07_BC、B、CRF01_AE、C、CRF08_BC5种亚型,其中CRF07_BC、B、CRF01_AE分别占32.6%、23.3%、20.9%。在同性性传播途径中,共存在CRF01_AE、CRF07_BC、B、CRF08_BC4种亚型,主要流行株均为CRF01_AE,占63.6%。在静脉吸毒者中,共发现CRF07_BC、B、CRF01_AE、A1、CRF02_AG5种亚型,CRF01_AE是该类人群的主要流行株,占58.4%。结论:流行于江苏省的HIV-1亚型种类较多;除CRF02_AG和A1亚型外,其余5种亚型均存在于性传播途径中;经性传播途径感染HIV-1可能是我省HIV-1亚型日趋复杂化的主要原因。

Objective：To analyze the subtypes distribution of Human Immunodeficiency Virus-1 （HIV-1）in Jiangsu province,and the relationship between subtypes and transmission route. Methods：83 blood samples with EDTA-K3 were collected from population with HIV-1 confirmed lately using western blot test. Viral RNA was extracted from plasma and used by a RT-nest PCR to amplify the env and gag gene of HIV-1. The PCR amplimers were partially sequenced on ABI 3730. After sequences alignmented by ClustalX, phylogenetic analysis was performed using MEGA 3.1 software. Results：7 subtypes including CRF01_AE, CRF07_BC,CRF08_BC, B,C,CRF02_AG,A1 were found circulating in Jiangsu. 3 predominated clades were CRF 01_AE,CRF07 BC,and B,which accounted for 31.6%, 25%, and 19.7%, respectively. 5 subtypes consisting of CRF07_BC, B, CRF01_AE, C, CRF08_BC, circulated in population via heterosexual contact, and CRF 07_BC accounted for 32.6%, B was 23.3%, and CRF 01_AE was 20.9%. CRF 01_AE, CRF 07_BC,B,CRF 08_BC circulated among men who sex with men having HIV-1 ,and CRF 01_AE was dominative. The circulated HIV-1 subtypes among IDUs included CRF 07_BC,B,CRF 01_ AE,A1,CRF 02_AG,CRF 01_AE was the dominated,which accounted for 58.4%. Conclusion：There were more kinds of subtypes circulating in Jiangsu than other province. Of 7 subtypes,5 except CRF02_AG and A1 were found in population with HIV-1 via heterosexual contact. The increasing complex characteristic of HIV-1 sequences may be associated with acquiring HIV via sexual intercourse.