C反应蛋白自身抗体与急性冠脉综合征斑块不稳定的相关性研究

A preliminary study between serum anti-CRP autoantibodies and plaque unstable in acute coronary syndrome

目的:探讨C反应蛋白(CRP)自身抗体与急性冠脉综合征(ACS)患者冠脉病变严重程度、斑块不稳定的相关性。方法:应用间接酶联免疫吸附法检测120例ACS,61例稳定型心绞痛(SAP)和120例对照者血清CRP自身抗体吸光度(A)值,并以对照组的95%可信区间A值作为判断CRP自身抗体阳性或阴性的界点。结果:ACS征组血浆CRP自身抗体A值水平(1.75±1.09)显著高于SAP(1.27±0.61)和对照组(0.88±0.43),P<0.001,并与冠状动脉病变积分无相关性(P>0.05)。ACS组CRP自身抗体阳性比例(44.17%)显著高于SAP组(8.84%),P<0.001。结论:CRP自身抗体不是反映ACS患者冠状动脉病变严重程度的指标,CRP自身抗体水平可能是随着斑块不稳定而变化的,与ACS患者斑块不稳定相关,但CRP自身抗体在ACS中的作用机制尚不明了。

Objective：To investigate the changes of serum anti-CRP autoantibodies（CRP-Ab） and their relationship in acute coronary syndrome （ACS） patients. Methods：Serum samples were taken from all patients with ACS（n=120）,stable angina pain （SAP）（n=61） and subjects without coronary heart disease （NCAD） （n=120）subjects. The serum levels of CRP-Ab（IgG）were quantitatively measured by indirect ELISA assay. Absorbance（A ） was measured at 405 nm. Positive cut-off values were calculated from the 95 th percentile of the A values in NCAD subjects. Results：The serum levels of CRP-Ab were greatly elevated in patients with ACS （1.75±1.09） compared with SAP（1.27±0.6.1） and NCAD（0.875±0.43） subjectes （P 〈 0.01 ,respectively）. The positive tatio of CRP-Ab in ACS group （44.17%） are higher than SAP group（8.84%） （P 〈 0.001 ）. There was no significant correlation between the serum level of CRP-Ab and the number of lesioned artery of coronary artery stenosis （P 〉 0.05）. Conclusion：we found that autoantibodies to CRP are com- mon in sera from patients with ACS. It is likely that this explains the relative of CRP response in patients with ACS.It can be exclud- ed that anti-CRP autoantibodies have biological potentials of pathophysiological interest in ACS.