附睾精子冷冻保存对单精子胞浆内注射临床结局的影响

The outcome of ICSI with frozen-thawed sperm obtained by PESA on azoospermia patients

目的:回顾性分析54例采用冷冻附睾精子行卵细胞浆内单精子注射(ICSI)的胚胎发育以及妊娠结局。方法:54例冷冻保存的附睾精子解冻复苏,采用ICSI法受精,观察胚胎发育形态,经72h体外培养后移植,将282例采用新鲜附睾精子的ICSI结局为对照组。结果:正常受精率、卵裂率、优质胚胎率、妊娠率冻精组与新鲜组之间无显著差异(72.4%vs74%,98.1%vs96.3%,51.7%vs46.8%,42.6%vs39.0%,P>0.05),两组均未出现新生儿畸形。冷冻附睾精子复苏率为65.9%。结论:冷冻附睾精子对胚胎发育以及妊娠结局无显著影响。

Objective：To investigate the effects of ICSI（intracytoplasmic sperm injection）cycles with frozen sperm obtained by PESA（percutaneous epididymal sperm aspiration）by retrospective study performed in 54 patients. Methods： Sperm from the 54 infertile males by PESA were frozen with conventional freezing method. After frozen-thawed and ICSI process,the fertilized oocytes were cultured in vitro for 72 hours and high quality embryos were transfered to the wives. 282 cases of fresh epidielymal sperms were tested as control. Fertilization rate,implantation rate,clinical pregnancy rate were compared beween two groups, the cycle with frozen-thawed sperm and the cycles with fresh PESA sperm as control. Results： No significant differences were found in rate of fertilization, cleavage high-qualified embryo,clinical pregnancy between two groups （72.4% vs 74%,98.1% vs 96.3%,51.7% vs 46.8%,42.6% vs 39.0%, respectively,P 〉 0.05）. Neither vital pregnant complications nor neonate malformations were found in both groups. Recovering rate of frozen sperm form PESA was 65.9%. Conclusion：The embryo development and pregnant outcome were not effected by using frozenthawed sperm obtained by PESA.