双波长RP-HPLC法同时测定茵陈蒿中绿原酸、咖啡酸和对羟基苯乙酮的含量

RP-HPLC simultaneous determination of chlorogenic acid,caffeic acid and 4-hydroxyacetophenone in herb of Artemisia capillaris Thunb. under different UV-vis wavelengths

目的:建立同时测定茵陈蒿中绿原酸、咖啡酸和对羟基苯乙酮含量的方法。方法:采用紫外-双波长同时检测的RP-HPLC法:C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.04%磷酸水溶液(9∶91),流速1.0mL·min-1,检测波长:325nm(绿原酸、咖啡酸);275nm(对羟基苯乙酮)。结果:绿原酸、咖啡酸和对羟基苯乙酮进样浓度分别在52.10～1.303×103μg·mL-1(r=0.9999)、1.756～43.91μg·mL-1(r=0.9999)、0.6574～16.43μg·mL-1(r=0.9995)范围内与峰面积呈良好的线性关系(n=5);方法回收率(n=9)分别为98.1%,98.6%,100.8%。结论:方法简便、快速、准确,重现性好,为茵陈蒿药材的质量控制提供依据。

Objective： To establish a method for the determination of chlorogenic acid, caffeic acid and 4 - hydroxyacetophenone in herb of Artemisia capillaris Thunb. simultaneously under different UV wavelengths. Methods：An RP- HPLC method detected by different UV wavelengths-325 nm for chlorogenic acid and caffeic acid,275 nm for 4 -hydroxyacetophenone respectively had been developed ：The system consisting of a C18 column （250 mm × 4.6 mm,5 μm ） and a mixture of acetonitrile and 0. 04% phosphate acid as the mobile phase was adopted;The flow rate was 1.0 mL · min^-1. Results： The linear response range was 52. 10 - 1. 303 × 103 μg · mL^-1 （r =0. 9999）, 1. 756 - 43.91 μg · mL^-1 （ r = 0. 9999 ） and 0. 6574 - 16.43 μg · mL^-1 （ r = 0. 9995 ）, respectively （ n = 5 ）. The average recoveries （ n = 9 ） of chlorogenic acid, caffeic acid and 4 - hydroxyacetophenone were 98.1%, 98.6% and 100. 8% , respectively. Conclusion：The assay demonstrates that the method has adequate accuracy and selectivity to measure the four chemical constituents in herb of Artemisia capillaris.