摘要
目的:建立高效液相色谱-质谱/质谱联用测定人血浆中赖诺普利浓度的方法,用于赖诺普利口腔崩解片的人体相对生物利用度和生物等效性研究。方法:使用Hypurity C18柱(2.1mm×150mm,5μm),以0.1%甲酸-乙腈(30∶70)为流动相,流速为0.2mL·min-1,柱温为40℃。以二级质谱选择离子反应监测方式(SRM)扫描检测。结果:赖诺普利的线性浓度范围为1.64~210ng·mL-1(r=0.9915),最小检出浓度为0.82ng·mL-1。提取回收率大于50%,平均相对回收率在80%~120%范围内。日内与日间变异均小于15%。结论:本法简便、灵敏,可用于赖诺普利的药物代谢动力学和生物等效性研究。
Objective: To establish a method of HPLC - MS/MS to detect lisinopril tablets in human plasma. Methods:Chromatographic column was Hypurity C18(2. 1 mm × 150 mm,5 μm) ;The mobile phase was 0. 1% formic acid - acetonitrile (30: 70) ;The flow rate was 0. 2 mL · min^-1 and temperature of column was 40℃. A MS/MS selective reaction monitoring mode was taken to quantitate lisinopril in human plasma. Results:Standard curve was linear in the range of 1.64 -210 ng · mL^-1 (r =0. 9915). The lower limit of detections of lisinopril was 0. 82 ng · mL^-1. The absolute recovery was more than 50% ,and the relative recoveries of lisinopril were in the range of 80% - 120%. Relative standard deviations of intraday and interday assays of lisinopril were both less than 15%. Condusion: The method is convenient and sensitive for detecting the concentration of lisinopril in human plasma, it can be used to study the pharmacokinetics and bioequivalence of lisinopril.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第6期931-934,共4页
Chinese Journal of Pharmaceutical Analysis