气相色谱法测定一次性使用输液器中环己酮的残留量

GC determination of residual cyclohexanone in disposable infusion set

目的:建立一次性使用输液器中环己酮残留量的测定方法。方法:利用蠕动泵对输液器中的环己酮进行提取,采用气相色谱法进行测定。色谱柱为HP-FFAP石英毛细管柱(30m×0.53mm×1.0μm);进样口温度200℃;柱温90℃;氢火焰离子化检测器(FID),检测器温度260℃;载气为氮气;流量1.7mL·min-1;分流比:5∶1;进样体积1.0μL;以无水乙醇为内标物质,以水为溶剂。结果:环己酮与内标物质在4min内分离良好;线性相关系数为0.9997;平均回收率为100.1%(n=9)。结论:该方法简单、快速、准确,可用于一次性使用输液器中环己酮残留量的检测。

Objective： To establish a method for the determination of residual cyclohexanone in disposable infusion set. Methods： Residual cyclohexanone in disposable infusion set was extracted with circular pump, then it was determined by GC with HP - FFAP capillary column, FID detector and using nitrogen as carrier gas. The injection temperature was 200 ℃, the column temperature was 90 ~C and the detector temperature was 260 ℃. The flow rate of nitrogen was 1.7 mL · min^-1. The split ratio was 5： 1. The injection volume was 1.0 μL. The internal standard was alcohol , and the solvent was water. Results：The cyclohexanone and internal standard were separated perfectly with 4 min. The correlation coefficient of the linear equation was O. 9997, the average recovery was 100. 1% （ n = 9）. Conclusion： The method is simple, rapid and accurate for the determination of residual cyclohexanone in disposable infusion set.