头孢拉定在玻碳电极上的电化学行为研究

Study on electrochemical behavior of cefradine at glassy carbon electrode

目的：应用线性扫描伏安法和循环伏安法研究了头孢拉定的电化学行为。结果：头孢拉定在pH=6.0的0.1 mol/L柠檬酸钠-氢氧化钠介质中,在＋0.23V（vs.Ag/AgC1）电位处产生一灵敏的阳极氧化峰,应用DPV法进行测定,该峰电流值ip与头孢拉定浓度在3.75×10^-5～1.5×10^-4mol/L范围内有良好的线性关系（r=0.9950）,据此建立了伏安法检测头孢拉定的方法,检出限为2.5×10^-6mol/L,样品测定平均加标回收率达97.50%,RSD=7.48%。结论：同时,研究发现头孢拉定的电极过程为受吸附控制的不可逆过程,电极反应转移电子数为1。

Objective ： The electrochemical behavior of cefradine has been studied by using linear sweep vohammetry and cyclic vohammetry. Results： In a medium of 0. 1 mol/L Sodium citrate - NaOH （ pH = 6. 0 ） , a sensitive oxidation peak of cefradine （CEP） was obtained by Glassy Carbon Electrode at the potential of ＋ 0. 23 V （ vs. Ag/AgC1 ）. And, by using DPV mensuration, a sound linear range between the peak current and the concentration of CEP was found to be maintained between 3.75 × 10^-5 1.5× 10^- 4 mol/L （r = 0. 9950）, on which the detection of cefradine by voltammetry was established. The detection limit was 21 5 × 10^-6 mol/L, with the recovery of CEP 97.50%, and RSD 7. 48%. Conclusion： The electrode reduction process of CEP at Glassy Carbon Electrode is irreversible with adsorptive characteristics and one electron is transferred in all electrode reaction.