钙指示剂羧酸钠-SDS体系共振瑞利散射法测定蛋白质

Determination of protein by resonance light scattering method after combining with falcon carboxylic acid SDS

目的：探索用于蛋白质定量分析增敏效果的方法。方法：研究发现在1.0 mol/L的硫酸介质中有十二烷基磺酸钠（SDS）,蛋白质与钙指示剂羧酸钠作用形成复合物,使最大波长350 nm的共振光散射光谱得到加强,根据其共振光散射的增强程度,可定量测定蛋白质,SDS的加入,使灵敏度提高3.5倍。结果：在选定条件下,几种蛋白质在0.008～25μg/m l浓度范围内与ΔI350nm呈线性关系,建立了定量测定蛋白质的新方法。结论：该法操作简便,灵敏度高,线性范围宽,重现性较好,用于人血清中总蛋白质的测定,结果与经典的考马斯亮蓝法一致。

Objective： A novel method for the determinnation of proteins by resonance light scattering （RLS） was developed. Methods ： It was based on the enhancement effect of SDS on the RLS intensity of the complex formed between protein and Calcon carboxylic acid in the presence of SDS at 1. 0 mol H2 SO4. The enhancement of RLS intensity was about 3.5 times greater than that in the absence of SDS. Results：h exhibits a resonance scattering peak at 350 nm ,and results in a great enhancement of RLS signal. Under the conditions, the concentration of the proteins in the range of 0. 008 - 25 μg/ml is linear to the △I350nm. The method has advantages of simple operation, high sensitivity, good accuracy and wide linear range. Conclusion：Proteins in human serum samples have been determined and the results obtained conformed quite well with coomassie brilliant blue G - 250 method.