两种快速检验流感病毒的方法比较

Comparison of two methods for rapid detection of influenza virus

目的:比较胶体金法和斑点酶免疫渗滤优缺点。方法:将4种亚型流感病毒培养液稀释后,用胶体金和斑点酶免疫渗滤两种方法同时进行快速检验。结果:胶体金法操作更简便快速,而斑点酶免疫渗滤法敏度更高。结论:在临床诊断中,斑点酶免疫渗滤更为实用有效,在样品量较大或现场处理样品时,用金标法进行筛检更方便。

Objective： Comparison of merits and limitations between colloidal gold colloid immunochromatography assay and dot immuno - enzyme filtration assay. Methods ： Four subtype of Influenza virus were diluted, then the dilutions were detected with two methods above. Results ： Colloidal gold colloid immunochromatography assay are more simpl and rapid than dot immuno - enzyme filtration assay, and the latter are more sensitive. Conclusion： Dot immuno - enzyme filtration assay are suitable in clinical diagnosis and colloidal gold colloid immunochromatography assay are convenient in field -test and the massive specimens.