副溶血弧菌TLH基因检测

Analysis of thermolabile hemolysin genotypes of Vibrio parahaemolyticus

目的:对副溶血弧菌(Vibrio parahaem olyticus,Vp)临床分离株中不耐热溶血素(thermolab ile hemolysin,TLH)基因进行检测,评价TLH基因在临床检测中的价值,为副溶血弧菌快速检测和新国标方法的建立打下基础。方法:用PCR方法对副溶血弧菌临床分离株的不耐热溶血素(TLH)溶血素基因进行检测。结果:所有副溶血弧菌菌株均可检测到TLH基因。结论:TLH基因在副溶血弧菌的检测中有一定的价值。

Objective ： To control contamination of Vibrio parahaemolyticus is often hampered by the lack of standardized methods and by the uncertainty associated with biochemical identification of the isolates. In this study, Vibrio parahaemolyticus was be examined thermolabile hemolysin （TLH） gene. Methods： PCR method was used to examine TLH gene isolates from clinical samples. Results：The TLH gene was found in all isolates. Condusion：TLH gene has some value in detecting Vibrio parahaemolyticus.