慢性乙型肝炎外周血单个核细胞和肝组织中IL-1β、IL-6的表达

EXPRESSION OF IL-1β AND IL-6 IN PERIPHERAL BLOOD MONONUCLEAR CELLS AND LIVER OF PATIENTS WITH CHRONIC HEPATITIS B

目的：进一步了解ＩＬ１β、ＩＬ６在慢性乙型肝炎发病过程中的作用。方法：本文采用斑点杂交方法对２０例慢性乙型肝炎患者和１０例健康人外周血单个核细胞中ＩＬ１β、ＩＬ６ｍＲＮＡ的表达水平进行了检测，同时运用ＡＢＣ免疫组化法对１３例慢性乙型肝炎患者肝组织及８例非肝病患者肝组织中ＩＬ１β、ＩＬ６的表达水平进行了检测。结果：慢性乙型肝炎患者外周血和肝组织中ＩＬ１β、ＩＬ６的表达水平均升高。结论：慢性乙型肝炎患者外周血及肝组织中ＩＬ１β、ＩＬ６的水平的升高可能发生在基因转录水平上，其与慢性乙型肝炎患者病变的发展和肝硬化的形成有着十分密切的关系。

Objective: In order to investigate the role of IL-1β and IL-6 in the pathogenesis of chronic hepatitis B. Methods: The expression of IL-1β and IL-6 mRNA in peripheral blood mononuclear cells (PBMC) of 20 patients with CAHB (chronic active hepatitis B) and 10 healthy donors were investigated by dot blot hybridization, while the expression of IL1β and IL6 were determined in the liver biopsy of 13 patients with CAHB and 8 nonhepatitis donors by ABC immunohistochemistry method. Results: Both the levels of IL-1β and IL-6 in PBMC and the liver of CAHB were higher than those of the healthy control. Conclusion: These results suggested that abnormal expression of IL-1β and IL-6 in patients with chronic hepatitis B might occur at the transcriptional level. It implicated in the pathogenesis and progression of chronic hepatitis B.