慢性肝病患者肝组织中HGV抗原的表达及意义

IMMUNOHISTOCHEMICAL DETECTION AND SIGNIFICANCE OF HEPATITIS G VIRUS ANTIGEN IN LIVER TISSUE OF PATIENTS WITH CHRONIC LIVER DISEASES

目的探讨慢性肝病患者组织中庚型肝炎病毒（ＨＧＶ）表达与意义。方法应用免疫组织化学方法以鼠抗ＨＧＶＮＳ５甲克隆抗体检测１４２例慢性肝病患者肝组织中ＨＧＶ抗原，部分患者采用ＲＴ－ＰＣＲ方法检测其血清中ＨＣＶＲＮＡ。结果１４２例肝病患者中，２９例（２０．４％）组织中检出ＨＧＶ抗原，肝硬化组（４２．９％，１２／２８）较慢性肝炎（１５．９％８／１１）和肝癌（１４．３％，９／６３）组检出率高。阳性信号位于胞浆中，阳性细胞可成散在、簇状或弥漫性分布。阳性细胞周围可有炎性坏死；肝癌患者抗原阳性细胞主要位于癌旁肝组织，癌巢中仅偶见少数散在分布的阳必细胞；绝大多数组织抗原阳性者其血清ＨＧＶＲＮＡ为阳性。有４例患者组织中ＨＧＶ抗原阳性但其血清ＨＧＶＲＮＡ阴性。结论ＨＧＶ可在慢性肝病包括肝细胞肝癌患者肝组织中表达，ＨＧＶ感染在慢性肝炎病期进展及肝癌发生中具有一定意义。

Objective To study the expression and its significance of hepatitis G virus (HGV) in liver tissue. Methods HGV antigen was detected with immunohistochemical method using moloclonal mouse antibody to HGV NS5 in 142 patients with chronic liver disease, and serum HGV RNA was detected by RT-nested PCR in some of the subjects. Results Out of 142 subjects, 29(20,4%) had been detected to have HGV antigen in their liver, the detection rate in liver cirrhosis patients ( l2/28. 42.9%) was higher than that in chronic hepatitis (8/11, 15.9%) and hepatocellular carcinoma (HCC, 9/63. l4.3%) ones.The staining signal was located in liver cells cytoplasm, and the positive cells distributed scatterly, clusterly or diflusely. Necroinflammation could be observed around thd HGV antigen positive cells. In HCC, HGV antigen was mainly detected in tissues adjacent to cancerous cells, only a very few cancerous cells had been occasionally detected with the antigen. Most of the patients who had HGV antigen in their liver had been detected with HGV RNA in their serum except 4 cases who had been detected with HGV antigen in liver but without HGV RNA in their serum. Conclusion These results showed that HGV could express in the liver tissue of patients with chronic liver diseases including HCC. and HGV might have some role in the progression of chronic hepatitis and HCC formation.