摘要
目的:观察阿米替林(amitriptyline,AM I)对SNI(spared nerve injury)大鼠脊髓谷氨酸转运体-1(glutamate transporter subtype 1,GLT-1)表达的影响。方法:体重180-200 g的雄性SD大鼠随机分为4组:对照组(A)、SNI模型组(B)、AM I组(C)、SNI模型+AM I组(D),经腹腔注射0.2m l生理盐水(A和B)、10mg/kg AM I(C和D),每日两次。术后1、3、5 d取各组大鼠L3-L6脊髓,检测GLT-1蛋白和mRNA表达改变,同时使用Von Frey纤维测量大鼠手术侧机械痛阈值(mechanicalpain threshold)改变。结果:与A组相比,B组大鼠脊髓内GLT-1蛋白和mRNA术后1 d均表达增高,但术后3、5 d逐渐降低,同时机械痛阈值随时间下降,C组大鼠GLT-1表达随时间逐渐增加,但是机械痛阈值无明显改变,D组大鼠术后GLT-1表达较A组明显增高,但不随时间变化,给药后3、5 d机械痛阈值显著高于B组。结论:AMI可以增加GLT-1蛋白表达,可能是治疗神经病理性疼痛的机制之一。
Objective: To investigate influence of amitriptyline on glutamate transporter subtype 1 (GLT- 1 ) in spinal cord of rats after spared nerve injury (SNI). Methods: Male Sprague-Dawley rats weighing 180 - 200 g were divided into four groups : control ( A), SNI ( B), amitriptyline (C) and SNI + amitriptyline (D) groups, which respectively received intraperitoneal injections of 0.2ml saline (A and B), 10mg/kg amitriptyline (C and D), bid. The L3 -6 segments of the spinal cord were isolated in 1,3 and 5 days after surgery. Expression of GLT-1 was determined by Western blot and RT-PCR. The mechanical pain threshold of the rats before and after the operation was determined with a Von Frey hair test. Re- sults : The expression of GLT-1 at both protein and mRNA level in group B was higher than that in control on day 1 after operation followed with a decrease in expression on day 3 and 5. A decreased rat mechanical pain threshold was showed in group B. In group C, the expression of GLT-1 increased gradually and there were no changes of the mechanical pain threshold. The expression of GLT-1 in group D was higher than that in control and did not change on days 1, 3 and 5. The mechanical pain threshold in group D was higher than that in group B on days 3 and 5 after operation. Conclusions: Amitriptyline could increase the expression of GLT-1 which may be one of its mechanisms in the treatment of neuropathie pain.
出处
《中国疼痛医学杂志》
CAS
CSCD
2009年第3期171-174,共4页
Chinese Journal of Pain Medicine