MMP-9、CD44、TGF-β_1在脑星形细胞瘤中应用研究

The Brain Star Polygon Cell Lump Immunity Set Turns a Clinical Application Research

目的MMP-9和CD44共同定位在细胞表面,且这种共同定位有助于CD44将MMP-9锚定于细胞表面并对胶原IV降解,促进肿瘤细胞的浸润。用可溶性CD44阻断CD44功能后即可使其丧失锚定MMP-9的作用。而被CD44锚定于细胞表面的MMP-9还可使无活性的TGF-β1前体活化,活化的TGF-β1则是新生血管生长所必需的生长因子。通过对CD44锚定的MMP-9及TGF-β1在脑星形细胞瘤中表达情况进行分析,对其表达意义进行研究,对CD44、MMP-9、TGF-β1在脑星形细胞瘤的浸润转移中的作用进行初步探讨,同时对CD44、MMP-9、TGF-β1相关性进行分析研究。方法共收集2006年郑州大学第一附属医院和许昌市中心医院手术切除的脑星形细胞瘤标本98例,应用免疫组化SP法检测肿瘤组织中CD44、MMP-9、TGF-β1的表达,分析CD44、MMP-9、TGF-β1和脑星形细胞瘤分级之间以及CD44、MMP-9、TGF-β1之间的关系。结果(1)CD44在星形细胞瘤组织中表达率77.55%(76/98),显著高于正常脑组织0(P<0.05)。通过Spearman等级相关检验,不同级别CD44表达具有统计学意义(r=0.95,P=0.046),星形细胞瘤级别与CD44阳性程度之间呈正相关。用Fishers精确检验法对不同级别进行检验,P=0.00027,说明不同级别之间CD44表达的构成比有差异性。(2)MMP-9在星形细胞瘤组织中表达率69.38%(68/98),显著高于正常脑组织(P<0.05)。通过Spearman等级相关检验,不同级别MMP-9表达具有统计学意义(r=0.89,P=0.038)。星形细胞瘤级别与MMP-9阳性程度之间呈正相关,用Fishers精确检验法对肿瘤4个级别MMP-9观察结果的构成情况进行检验,P=1.12×10-7。(3)TGF-β1在星形细胞瘤组织中表达率74.481%(73/98),显著高于正常脑组织(P<0.05)。通过Spearman等级相关检验,不同级别TGF-β1表达具有统计学意义(r=0.91,P=0.046)。星形细胞瘤级别与TGF-β1阳性程度之间呈正相关。用Fishers精确检验法对肿瘤4个级别TGF-β1观察结果的构成情况进行检验P=0.0001。(4)星形细胞瘤中CD44、MMP-9、TGF-β1表达的相关性分析CD44表达阳性的病例中,MMP-9的阳性表达率为94.12%,CD44表达阴性的病例中,MMP-9的阴性表达率为60.00%,CD44的表达和MMP-9表达之间呈正相关关系(列联系数rp=0.496016,P=1.559×10-8)。CD44表达阳性的病例中,TGF-β1的阳性表达率为82.89%,CD44表达阴性的病例中,TGF-β1的阴性表达率为54.55%,CD44的表达与TGF-β1表达呈正相关关系(列联系数rp=0.3136,P=0.001076)。MMP-9表达阳性的病例中,TGF-β1的阳性表达率为91.17%,MMP-9表达阴性的病例中,TGF-β1的阴性表达率为63.33%,经统计学分析表明MMP-9表达与TGF-β1表达呈正相关关系(列联系数rp=0.4825,P=4.93×10-8)。结论联合检测MMP-9、CD44、TGF-β1三者对脑星形细胞瘤的预后判断有重要意义。

Objective MMP-9 and CD44 together position in the cell surface,and this kind of together positions to help the CD44 to settle anchor roans MMP-9s to also decline a solution to the gum original Ⅳ at the cell surface and promote tumor cell gradually. Stopping the empress of the function of CD44 with the dissolubility CD44 can immediately make it lose the function that the anchor man settles MMP-9. But is settle by the CD44 anchor manses superficial at the cell of the MMP-9 can also make TGF-β1 of have no activity-TGF-β1 ex- bodies activate and activate of TGF-β1 new-born blood vessel growth is the essential growth factor. This text passes the MMP-9 and TGF-β1 which settles to the CD44 anchor manses-TGF-β1 the expression circumstance carries on analysis in the brain star polygon cell lump,as to it＇s express meaning to carry on a research,to the CD44,MMP-9,TGF-β1 gradually transfer a medium function to carry on a first step study in the brain star polygon cell lump,in the meantime to the CD44,MMP-9,TGF-β1 relativities carry on an analytical research. Methods Totally collect 2006 subsidiary hospital of Zheng4 Zhou＇s university first and Xu3 Chang hospital surgical operation in the city center excision of the brain star polygon cell lump the specimen be 98 and apply an immunity set to turn the SP method examination tumor the CD44,MMP-9,TGF-β1 in the organization-TGF-β1 expression,analytical CD44,MMP-9,TGF-β1 with and CD44,MMP-9,TGF-β1 of the brain star polygon cell lump ratings-TGF-β1. The relation of one its. Results （1）CD44 the expression rate is 77.55%（76/98） in the star polygon cell lump organization and show Zhao Gao to organize 0 at the normal brain （P〈0.05）. Pass the related examination of the Spearman grade,different Class CD44 expression r=0.95,P=-0.046, have covariance to learn meaning,the of the star polygon cell lump Class and the CD44 male degree present just related. Use the Fishers precision examination method to carry on an examination to the different Class,P=0.00027,the composing ratio which explains an of different Class CD44 expressions contains difference. （2）MMP-9 the expression rate is 69.38%（68/98） in the star polygon cell lump organization and show Zhao Gao to organize at the normal brain （P〈0.05）. Pass the related examination of the Spearman grade, different Class MMP-9 expression r=0.89,P=-0.038,have covariance to learn meaning.The of the star polygon cell lump Class and the MMP-9 male degree present just related,the composing circumstance which uses the Fishers precision examination method 4 observation result of the Class MMP-gs to the tumor carries on an examination,P=-1.12 × 10^-7. （3）TGF-β1 the expression rate is 74.481%（73/98） in the star polygon cell lump organization and show Zhao Gao to organize at the normal brain （P〈0.05）. Pass the related examination of the Spearman grade,different Class TGF-β1 expression r=-0.91,P=0.046,have covariance to leam meaning. The star polygon cell lump Class and TGF-β1 of male degrees present just related.Use the Fishers precision examination method 4 Class TGF-β1 to the tumor-TGF-β1 composing circumstance of observation result carry on an examination,P=-0.0001. （4）Star polygon celt CD44,MMP-9,TGF in lump-TGF-β1 expression of relativity analysis CD44 expression masculine gender of in the case,the male expression rate of MMP-9 is 94.12%,CD44 expression feminine gender of in the case,the feminine gender expression of the MMP-9 rate is 60.00%, of the expression and the expressions MMP-9s of CD44 presents a just related relation （the row contact few rp=0. 496016, P=-1.559 × 10^-8）. The CD44 expresses a male case in,TGF-β1 male expression rates is 82.89% ,in the case of the CD44 expression feminine gender,TGF-β1 feminine gender expression rates is 54.55%, the expression and TGF-β1 of CD44-TGF-β1 expressions present a just related relation （the row contact few rp=0.3136, P = 0.001076）. The MMP-9 expresses a male case in, TGF-β1 male expression rates is 91.17%,in the case of the MMP-9 expression feminine gender,TGF-β1 feminine gender expression rates is 63.33%,Be learned analysis the enunciation MMP-9 to express by covariance and TGF-β1 expressions present a just related relation （the row contact few r,=0.4825,P=4.93 × 10^-8）. Conclusion The detecdion of MMP-9,CD44 and TGF- β1 is significance for prognosis of the brain star polygon cell lump.