药物对提高人胰岛素基因在糖尿病大鼠体内表达效能的研究

Research about Expression Efficacy of Human Insulin Gene in Type 1 Diabetes Rat Enhanced by Medicine

目的本实验通过探索药物对胰岛素基因表达效能的影响,以期提高以逆转录病毒为载体进行糖尿病基因治疗的效能,为该方法治疗糖尿病的临床实施缩短距离。方法建立链脲佐菌素诱导的1型糖尿病大鼠模型,对含有目的基因的重组质粒转染组给予肝细胞生长因子(HGF)、3碘甲状腺原氨酸(T3),以不加干预措施的转染组为对照,观察各组大鼠血糖的控制情况和胰岛素表达水平。结果经腹腔导入含有目的基因的重组质粒同时给予HGF、T3组的胰岛素基因表达效果最佳,仅注入重组质粒组的基因表达有轻度改善,仅注入HGF、T3组和糖尿病对照组表达无改善。结论在动物实验中通过观测各组大鼠胰岛素及血糖水平和体重的变化表明,应用HGF、T3后可明显提高含有目的基因的重组质粒的转染和整合效率。该实验为基因治疗糖尿病的临床实验奠定基础。

Objective To enhance the efficacy of gene therapy for diabetes mellitus and remove the obstacles for the clinical application, this experiment explores different medicines. Methods Type 1 diabetes mellitus model of rat is established by STZ. After that,we injected HGF and T3 to one experimental groups, but do not take any measures to the other experimental group. After the plasmids are injected we observe the level of blood glucose and plasma insulin. Results The group treated with the reorganization plasmids including goal gene which were perfused into the abdominal cavity and HGF and T3 injected 24 hours ago have the best effect. The group treated with only the reorganization plasmids including goal gene which were perfused into the abdominal cavity have slight effect. The group given only HGF and T3 and the group with no treatment have not any effect. Conclusion In animal experiment, through observing the level of insulin and glucose and the changes of weight we find that injecting HGF and T3 can obviously enhance the transfer and integration efficiency of reorganization plasmids. So this experiment lays the foundation for the clinical experiment.