急性心肌梗塞血小板中PKC底物磷酸化程度的实验研究

Experimental Study on the Phosphorylated Protein Kinase C Substrate of Platelet in Acute Myocardial Infarction

目的：研究急性心肌梗塞（ＡＭＩ）时脂蛋白（ａ）［ＬＰ（ａ）］，血小板（ＰＬＴ）及蛋白激酶Ｃ（ＰＫＣ）的关系。方法：分别以高ＬＰ（ａ）血症的急性心肌梗塞（ＡＭＩ）病人及健康对照者为对象，检测其ＰＬＴ及ＰＬＴ中ＰＫＣ底物４０ｋｄ蛋白磷酸化程度，对检测结果进行统计学分析。结果：ＡＭＩ组ＰＬＴ数目及其４０ｋｄ蛋白磷酸化程度明显高于对照组。并且，磷酸化程度随时间延长而增强。结论：高ＬＰ（ａ）血症的ＡＭＩ患者ＰＬＴ数目及ＰＬＴ中ＰＫＣ底物４０ｋｄ蛋白磷酸化程度均增高。

Objective: We studied the relation of lipoprotein (a) 、platelet (PLT) and protein kinase C (PKC) in acute myocardial infarction (AMI). Methods: We tested PLT and 40 kd protein phosphorylated of PKC substrate in PLT for AMI with high plasma LP(a) concentration and for thecontrol group. Results: The number of PLT and the level of 40 kd protein phosphorylated of PKC substrate in experimental group were obviously higher than those in control group. The level of 40 kd protein phosphorylated of PKC substrate rose with the prolongation of time. Conclusions: The number of PLT and the level of 40 kd protein phosphorylated of PKC substrate in the AMI with hight plasma LP(a) concentration increased. 